Pseudomonas aeruginosa is a serious opportunistic pathogen and one of many main bacterial species inflicting well being care-associated infections. Carbapenems are the simplest antimicrobial brokers for the remedy of extreme infections attributable to P. aeruginosa Nevertheless, our current surveillance demonstrated that the prevalence of carbapenem-resistant P. aeruginosa (CRPA) reached 38.67% in Zhejiang, China.
By analyzing CRPA isolates collected from sufferers from 2006 to 2018, we discovered that 33% of CRPA isolates carried the gene bla KPC-2, which conferred high-level resistance to carbapenems and different β-lactams. Particularly, a CRPA clone, ST463 (sequence sort 463), emerged and has turn out to be the predominant CRPA clone among the many inhabitants. Genome sequencing demonstrated that ST463 enlargement was related to plasmid-borne bla KPC-2 The cellular factor flanking bla KPC-2, the kind IV secretion system, and the profitable enlargement of clone ST463 might need additional favored bla KPC-2 unfold in P. aeruginosa Molecular clock evaluation dated the emergence of clone ST463 to round 2007.
Genome-wide affiliation evaluation confirmed that 567 genes have been related to clone ST463, together with a number of identified virulence genes associated to the biosynthesis of lipooligosaccharide (LOS) O-antigens and exotoxin. These findings point out that ST463 is increasing with plasmid-borne bla KPC-2 and virulence-related genes in nosocomial infections, and shut surveillance ought to be undertaken sooner or later.
IMPORTANCE Well being care-associated infections, often known as nosocomial infections, are essentially the most frequent hostile occasions in well being care supply worldwide, inflicting excessive charges of morbidity and mortality and excessive well being care prices. Pseudomonas aeruginosa is among the main bacterial species inflicting well being care-associated infections. Carbapenems are the simplest antimicrobial brokers for the remedy of its extreme infections. Nevertheless, the prevalence of carbapenem-resistant P. aeruginosa (CRPA) has been growing quickly in recent times, and our surveillance demonstrated that the prevalence of CRPA reached 38.67% in Zhejiang, China. Genome sequencing of CRPA isolates over a decade confirmed {that a} CRPA clone (ST463) emerged not too long ago.
The clone is extremely proof against β-lactams, together with carbapenems, and fluoroquinolones. Genome-wide affiliation evaluation confirmed that the clone expanded with virulence-related genes and the plasmid-borne carbapenem-resistant gene bla KPC-2 These findings are of great public well being significance, as the data will facilitate the management and minimization of CRPA nosocomial infections.
Full genome sequence of marine Roseobacter lineage member Monaibacterium sp. ALG8 with six plasmids remoted from seawater round brown algae
Monaibacterium sp. ALG8 (=MCCC 1 Okay04733) was remoted from seawater round brown algae. The genome of Monaibacterium sp. ALG8 was sequenced, one round 3,036,380 bp chromosome and 6 round plasmids starting from 12,229 to 151,263 bp have been discovered after meeting. The outcomes of genomic annotation confirmed that Monaibacterium sp. ALG8 lacks the flexibility to degrade alginate, indicating its ecological position might not be immediately associated to the degradation of brown algae.
The comparability of genomic options within the plasmids confirmed that the majority of those plasmids, besides pALG4, have been horizontally recruited from donors, not ancestors. Based mostly on predicted capabilities, the existence of plasmids might present pressure ALG8 with benefits together with nitrate discount, tolerance of osmotic stress by way of glycine betaine, resistance to heavy steel stress equivalent to mercury and cobalt, degradation of benzoate metabolites equivalent to p-cumate, transformation of the swim-or-stick life-style and enchancment of the immune system with two CRISPR-Cas methods. This examine gives proof for the carbon metabolic patterns of Monaibacterium sp. ALG8 and predicts the capabilities and donors of six plasmids on this pressure, broadening our understanding of the ecological roles of micro organism within the setting round brown algae and the capabilities and evolutionary patterns of plasmids in marine Roseobacter lineage members.
A high-efficiency methodology for site-directed mutagenesis of huge plasmids primarily based on giant DNA fragment amplification and recombinational ligation
Web site-directed mutagenesis for big plasmids is a troublesome job that can’t simply be solved by the standard strategies utilized in many laboratories. On this examine, we developed an efficient methodology for Web site-directed Mutagenesis for Massive Plasmids (SMLP) primarily based on a PCR approach. The SMLP methodology combines a number of efficient approaches, together with a high-efficiency DNA polymerase for the big DNA amplification, two unbiased PCR reactions and a quick recombinational ligation.
 Recombinant HIV-2 Protease Protein, Untagged, E.coli-10ug |
|
QP12271-10ug |
EnQuireBio |
10ug |
EUR 201 |
 Recombinant Human BMP-2 Protein, Untagged, E.coli-10ug |
|
QP5363-10ug |
EnQuireBio |
10ug |
EUR 237 |
 Recombinant Porcine IL 2 Protein, Untagged, E.coli-10ug |
|
QP10699-10ug |
EnQuireBio |
10ug |
EUR 201 |
 Recombinant Canine MCP 2 Protein, Untagged, E.coli-10ug |
|
QP10782-10ug |
EnQuireBio |
10ug |
EUR 201 |
 Recombinant Human Arginase-2, GST, E.coli-10ug |
|
QP5676-ec-10ug |
EnQuireBio |
10ug |
EUR 200 |
 Recombinant Human Glutaredoxin-2, GST, E.coli-10ug |
|
QP8154-ec-10ug |
EnQuireBio |
10ug |
EUR 200 |
 Recombinant Human CXCL2/ MIP-2 Protein, Untagged, E.coli-10ug |
|
QP1016-10ug |
EnQuireBio |
10ug |
EUR 237 |
 Recombinant Influenza Parainfluenza Type-2 Protein, Untagged, E.coli-10ug |
|
QP12960-10ug |
EnQuireBio |
10ug |
EUR 155 |
 Recombinant Mouse MCP-2/ CCL8 Protein, Untagged, E.coli-10ug |
|
QP5471-10ug |
EnQuireBio |
10ug |
EUR 237 |
 Recombinant Human MMP-2 Protein, His, HEK 293-10ug |
|
QP10790-10ug |
EnQuireBio |
10ug |
EUR 201 |
 Recombinant Human STC 2 Protein, Untagged, HEK 293-10ug |
|
QP13613-10ug |
EnQuireBio |
10ug |
EUR 201 |
 Recombinant Bovine FGF 2 Protein, Untagged, Native Protein-10ug |
|
QP10599-10ug |
EnQuireBio |
10ug |
EUR 355 |
 Recombinant Human Semenogelin-2 Protein, His, Yeast-10ug |
|
QP9386-ye-10ug |
EnQuireBio |
10ug |
EUR 308 |
 Recombinant Human Hyaluronidase-2 Protein, His, Yeast-10ug |
|
QP9753-ye-10ug |
EnQuireBio |
10ug |
EUR 236 |
 Recombinant Human Ficolin-2 Protein, His, Yeast-10ug |
|
QP9759-ye-10ug |
EnQuireBio |
10ug |
EUR 236 |
 Recombinant Zebrafish Metallothionein-2 Protein, His, Yeast-10ug |
|
QP9826-ye-10ug |
EnQuireBio |
10ug |
EUR 362 |
 Recombinant Mouse Arginase-2, His-SUMO, E.coli-10ug |
|
QP5677-ec-10ug |
EnQuireBio |
10ug |
EUR 272 |
 Recombinant Mouse Bcl-2 Protein, His, E.coli-10ug |
|
QP5710-ec-10ug |
EnQuireBio |
10ug |
EUR 155 |
 Recombinant Bovine FGF 2 Protein, Untagged, E.coli-10ug |
|
QP10599-EC-10ug |
EnQuireBio |
10ug |
EUR 155 |
 Recombinant Human Plastin-2 Protein, His, Yeast-10ug |
|
QP6289-ye-10ug |
EnQuireBio |
10ug |
EUR 236 |
 Recombinant Human Galectin-2 Protein, GST, E.coli-10ug |
|
QP6295-ec-10ug |
EnQuireBio |
10ug |
EUR 200 |
 Recombinant Human Metaxin-2 Protein, GST, E.coli-10ug |
|
QP6393-ec-10ug |
EnQuireBio |
10ug |
EUR 272 |
 Recombinant Human Twinfilin-2 Protein, GST, E.coli-10ug |
|
QP7777-ec-10ug |
EnQuireBio |
10ug |
EUR 200 |
 Recombinant Human Prohibitin-2 Protein, GST, E.coli-10ug |
|
QP8027-ec-10ug |
EnQuireBio |
10ug |
EUR 200 |
 Recombinant Human Talin-2 Protein, His, Yeast-10ug |
|
QP8291-ye-10ug |
EnQuireBio |
10ug |
EUR 236 |
 Recombinant Chicken Vitellogenin-2 Protein, His, E.coli-10ug |
|
QP8859-ec-10ug |
EnQuireBio |
10ug |
EUR 326 |
 Recombinant Mouse Renin-2 Protein, His, E.coli-10ug |
|
QP8886-ec-10ug |
EnQuireBio |
10ug |
EUR 272 |
 Recombinant Human Metallothionein-2 Protein, GST, E.coli-10ug |
|
QP8894-ec-10ug |
EnQuireBio |
10ug |
EUR 200 |
 Recombinant E.coli Thioredoxin-2 Protein, His, Yeast-10ug |
|
QP7012-ye-10ug |
EnQuireBio |
10ug |
EUR 362 |
 Recombinant Mouse Thrombospondin-2 Protein, His, E.coli-10ug |
|
QP6786-ec-10ug |
EnQuireBio |
10ug |
EUR 272 |
 Recombinant Mouse Thrombospondin-2 Protein, His, Yeast-10ug |
|
QP6786-ye-10ug |
EnQuireBio |
10ug |
EUR 272 |
 Recombinant Human IGF-2/ IGF-II Protein, Untagged, E.coli-10ug |
|
QP5264-10ug |
EnQuireBio |
10ug |
EUR 136 |
 Recombinant Dengue Virus Dengue Envelope-2 Protein, Untagged, Insect-10ug |
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QP11626-10ug |
EnQuireBio |
10ug |
EUR 201 |
 Recombinant Human CCL24/ Eotaxin-2/ MPIF-2 Protein, His, E.coli-10ug |
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QP8522-ec-10ug |
EnQuireBio |
10ug |
EUR 200 |
 Protein, His, E.coli-10ug) Recombinant Human Bcl 2 (minus BH4 domain) Protein, His, E.coli-10ug |
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QP11138-10ug |
EnQuireBio |
10ug |
EUR 201 |
 Recombinant Human 2-5A-dependent ribonuclease Protein, His-SUMO, E.coli-10ug |
|
QP6997-10ug |
EnQuireBio |
10ug |
EUR 155 |
 Recombinant Bovine Serpin A3-2 Protein, His, Yeast-10ug |
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QP9699-ye-10ug |
EnQuireBio |
10ug |
EUR 362 |
 Recombinant Human SerpinB2/ PAI-2 Protein, Untagged, E.coli-10ug |
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QP10161-ec-10ug |
EnQuireBio |
10ug |
EUR 290 |
 Recombinant Human TIMP2/ TIMP-2 Protein, Untagged, E.coli-10ug |
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QP10162-ec-10ug |
EnQuireBio |
10ug |
EUR 290 |
 Recombinant Human CCL8/ MCP-2 Protein, Untagged, E.coli-10ug |
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QP10231-ec-10ug |
EnQuireBio |
10ug |
EUR 290 |
 Recombinant Human IL2/ Interleukin-2 Protein, Untagged, E.coli-10ug |
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QP10309-ec-10ug |
EnQuireBio |
10ug |
EUR 154 |
 Recombinant Human IGF1 Isoform 2 Protein, Untagged, E.coli-10ug |
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QP10390-ec-10ug |
EnQuireBio |
10ug |
EUR 154 |
 Recombinant Human CCL8/ MCP-2 Protein, GST, E.coli-10ug |
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QP4994-ec-10ug |
EnQuireBio |
10ug |
EUR 200 |
 Recombinant Human CCL8/ MCP-2 Protein, His, Yeast-10ug |
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QP4994-ye-10ug |
EnQuireBio |
10ug |
EUR 236 |
 Recombinant Human BMP-2 Protein, Untagged, HEK 293-10ug |
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QP5363-HEK-10ug |
EnQuireBio |
10ug |
EUR 218 |
 Recombinant Human Caspase-2 Protein, His-SUMO, E.coli-10ug |
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QP5762-ec-10ug |
EnQuireBio |
10ug |
EUR 200 |
 Recombinant Mouse Desmoglein-2 Protein, His-SUMO, E.coli-10ug |
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QP5947-ec-10ug |
EnQuireBio |
10ug |
EUR 326 |
 Recombinant Human Elongation factor 2 Protein, His, E.coli-10ug |
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QP5962-ec-10ug |
EnQuireBio |
10ug |
EUR 200 |
 Recombinant Human Estrogen Receptor 2 Protein, His, Yeast-10ug |
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QP6000-ye-10ug |
EnQuireBio |
10ug |
EUR 236 |
 Recombinant Mouse Hyaluronan synthase 2 Protein, His, E.coli-10ug |
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QP6144-ec-10ug |
EnQuireBio |
10ug |
EUR 272 |
 Recombinant Mouse Hyaluronan synthase 2 Protein, His, Yeast-10ug |
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QP6144-ye-10ug |
EnQuireBio |
10ug |
EUR 272 |
 Recombinant Human IL2/ Interleukin-2 Protein, GST, E.coli-10ug |
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QP6216-ec-10ug |
EnQuireBio |
10ug |
EUR 200 |
 Recombinant Human IL2/ Interleukin-2 Protein, His, Yeast-10ug |
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QP6216-ye-10ug |
EnQuireBio |
10ug |
EUR 236 |
 Recombinant Rabbit IL2/ Interleukin-2 Protein, His, E.coli-10ug |
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QP6218-ec-10ug |
EnQuireBio |
10ug |
EUR 326 |
 Recombinant Human Integrin alpha-2 Protein, His, Yeast-10ug |
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QP6238-ye-10ug |
EnQuireBio |
10ug |
EUR 236 |
 Recombinant Human Plastin-2 Protein, His-SUMO, E.coli-10ug |
|
QP6289-ec-10ug |
EnQuireBio |
10ug |
EUR 200 |
 Recombinant Human 2-oxoglutarate dehydrogenase, His-SUMO, E.coli-10ug |
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QP6447-ec-10ug |
EnQuireBio |
10ug |
EUR 200 |
 Recombinant Rat Neuroendocrine convertase 2 Protein, His, E.coli-10ug |
|
QP6473-ec-10ug |
EnQuireBio |
10ug |
EUR 326 |
 Recombinant Rat SerpinB2/ PAI-2 Protein, His, Yeast-10ug |
|
QP6669-ye-10ug |
EnQuireBio |
10ug |
EUR 272 |
 Recombinant Human Septin-2 Protein, His-SUMO, E.coli-10ug |
|
QP7552-ec-10ug |
EnQuireBio |
10ug |
EUR 272 |
 Recombinant Human Clavesin-2 Protein, His-SUMO, E.coli-10ug |
|
QP7684-ec-10ug |
EnQuireBio |
10ug |
EUR 200 |
 Recombinant Mouse 2-oxoglutarate dehydrogenase, His-SUMO, E.coli-10ug |
|
QP7717-ec-10ug |
EnQuireBio |
10ug |
EUR 272 |
 Recombinant Human Sesquipedalian-2 Protein, His-SUMO, E.coli-10ug |
|
QP7760-ec-10ug |
EnQuireBio |
10ug |
EUR 200 |
 Recombinant Human Complexin-2/ CPLX2 Protein, GST, E.coli-10ug |
|
QP7773-ec-10ug |
EnQuireBio |
10ug |
EUR 272 |
 Recombinant Human Vasohibin-2 Protein, His-SUMO, E.coli-10ug |
|
QP7784-ec-10ug |
EnQuireBio |
10ug |
EUR 272 |
 Recombinant Macaque CXCL10/ Crg-2 Protein, His, E.coli-10ug |
|
QP7849-ec-10ug |
EnQuireBio |
10ug |
EUR 326 |
 Recombinant Human Thioredoxin-2/ TXN2 Protein, GST, E.coli-10ug |
|
QP8009-ec-10ug |
EnQuireBio |
10ug |
EUR 200 |
 Recombinant Human Calponin-2 Protein, His-SUMO, E.coli-10ug |
|
QP8036-ec-10ug |
EnQuireBio |
10ug |
EUR 200 |
 Recombinant Human Thioredoxin reductase 2, His-SUMO, E.coli-10ug |
|
QP8200-ec-10ug |
EnQuireBio |
10ug |
EUR 200 |
 Recombinant Human Talin-2 Protein, His-SUMO, E.coli-10ug |
|
QP8291-ec-10ug |
EnQuireBio |
10ug |
EUR 200 |
 Recombinant Human Angiopoietin-2/ ANG2 Protein, His, E.coli-10ug |
|
QP8512-ec-10ug |
EnQuireBio |
10ug |
EUR 200 |
 Recombinant Human TIMP2/ TIMP-2 Protein, GST, E.coli-10ug |
|
QP8571-ec-10ug |
EnQuireBio |
10ug |
EUR 200 |
 Recombinant Mouse CXCL2/ MIP-2 Protein, His, E.coli-10ug |
|
QP8651-ec-10ug |
EnQuireBio |
10ug |
EUR 272 |
 Recombinant Human Glutamate carboxypeptidase 2 Protein, His, E.coli-10ug |
|
QP8726-ec-10ug |
EnQuireBio |
10ug |
EUR 200 |
 Recombinant E.coli GTP cyclohydrolase-2 Protein, His, E.coli-10ug |
|
QP8879-ec-10ug |
EnQuireBio |
10ug |
EUR 326 |
 Recombinant Mouse Angiopoietin-2/ ANG2 Protein, His, E.coli-10ug |
|
QP8884-ec-10ug |
EnQuireBio |
10ug |
EUR 272 |
 Recombinant E.coli Thioredoxin-2 Protein, His-SUMO, E.coli-10ug |
|
QP7012-ec-10ug |
EnQuireBio |
10ug |
EUR 326 |
 Recombinant Human Retinal dehydrogenase 2 Protein, His, Yeast-10ug |
|
QP8968-ye-10ug |
EnQuireBio |
10ug |
EUR 308 |
 Recombinant Mouse Elongation factor 2 Protein, His, Yeast-10ug |
|
QP9112-ye-10ug |
EnQuireBio |
10ug |
EUR 272 |
 Recombinant Human MBL-2/ MBL Protein, His, Yeast-10ug |
|
QP9268-ye-10ug |
EnQuireBio |
10ug |
EUR 236 |
 Recombinant Human Tryptase beta-2 Protein, His, E.coli-10ug |
|
QP6832-ec-10ug |
EnQuireBio |
10ug |
EUR 200 |
 Recombinant Mouse Tryptase beta-2 Protein, GST, E.coli-10ug |
|
QP6833-ec-10ug |
EnQuireBio |
10ug |
EUR 272 |
 KRTAP4-2 cloning plasmid |
|
CSB-CL861178HU-10ug |
Cusabio |
10ug |
EUR 233 |
- Formulation: 10 μg plasmid + 200μl Glycerol
- Length: 411
- Sequence: ATGGTCAACTCCTGTTGTGGCTCTGTGTGCTCTGACCAGGGCTGTGGCCTAGAGAACTGCTGCCGTCCCAGCTGCTGCCAGACCACCTGCTGCAGGACCACCTGCTGCCGCCCCAGCTGCTGTGTGTCCAGCTGCTGCAGACCGCAGTGCTGCCAGTCTGTGTGCTGCCAGCCCAC
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Description: A cloning plasmid for the KRTAP4-2 gene. |
 KRTAP3-2 cloning plasmid |
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CSB-CL871617HU-10ug |
Cusabio |
10ug |
EUR 233 |
- Formulation: 10 μg plasmid + 200μl Glycerol
- Length: 297
- Sequence: atggattgctgtgcctctcgcagctgcagtgtccccactgggcctgccaccaccatctgctcctccgacaaatcctgccgctgtggagtctgcctgcccagcacctgcccacacacagtttggttactggagcccatctgctgtgacaactgtcccccaccctgccacattcctca
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Description: A cloning plasmid for the KRTAP3-2 gene. |
 KRTAP9-2 cloning plasmid |
|
CSB-CL880139HU1-10ug |
Cusabio |
10ug |
EUR 257 |
- Formulation: 10 μg plasmid + 200μl Glycerol
- Length: 525
- Sequence: ATGACCCACTGTTGCTCCCCTTGCTGTCAGCCTACATGCTGCAGGACCACCTGCTGCAGGACCACCTGCTGGAAGCCCACCACTGTGACCACCTGCAGCAGCACATCCTGCTGCCAGCCCGCCTGCTGTGTGTCCAGCTGCTGCCAGCCTTGCTGCCGCCCAACTTCCTGTCAAAA
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Description: A cloning plasmid for the KRTAP9-2 gene. |
KRTAP9-2 cloning plasmid |
|
CSB-CL880139HU2-10ug |
Cusabio |
10ug |
EUR 257 |
- Formulation: 10 μg plasmid + 200μl Glycerol
- Length: 525
- Sequence: atgacccactgttgctccccttgctgtcagcctacctgctgcaggaccacctgctgcaggaccacctgctggaagcccaccactgtgaccacctgcagcagcacaccctgctgccagcccgcctgctgtgtgtccagctgctgccagccttgctgccgcccaacttgctgtcaaaa
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Description: A cloning plasmid for the KRTAP9-2 gene. |
Utilizing this methodology, we’ve got achieved quite a lot of mutants for the filamin A gene (7.9 kb) cloned within the pcDNA (5.four kb) or the pLV-U6-CMV-EGFP (9.four kb) plasmids, indicating that this methodology could be utilized to site-directed mutagenesis for the plasmids as much as 17.Three kb. We present that the SMLP methodology has a higher benefit than the standard strategies examined on this examine, and this methodology could be utilized to substitution, deletion, and insertion mutations for each giant and small plasmids in addition to the meeting of three fragments from PCR reactions. Altogether, the SMLP methodology is straightforward, efficient, and useful to the laboratories that require finishing the mutagenesis of huge plasmids.
