Emergence and Expansion of a Carbapenem-Resistant Pseudomonas aeruginosa Clone Are Associated with Plasmid-Borne bla KPC-2 and Virulence-Related Genes

Emergence and Expansion of a Carbapenem-Resistant Pseudomonas aeruginosa Clone Are Associated with Plasmid-Borne bla KPC-2 and Virulence-Related Genes

beads
Lieven0
Pseudomonas aeruginosa is a serious opportunistic pathogen and one of many main bacterial species inflicting well being care-associated infections. Carbapenems are the simplest antimicrobial brokers for the remedy of extreme infections attributable to P. aeruginosa Nevertheless, our current surveillance demonstrated that the prevalence of carbapenem-resistant P. aeruginosa (CRPA) reached 38.67% in Zhejiang, China.
By analyzing CRPA isolates collected from sufferers from 2006 to 2018, we discovered that 33% of CRPA isolates carried the gene bla KPC-2, which conferred high-level resistance to carbapenems and different β-lactams. Particularly, a CRPA clone, ST463 (sequence sort 463), emerged and has turn out to be the predominant CRPA clone among the many inhabitants. Genome sequencing demonstrated that ST463 enlargement was related to plasmid-borne bla KPC-2 The cellular factor flanking bla KPC-2, the kind IV secretion system, and the profitable enlargement of clone ST463 might need additional favored bla KPC-2 unfold in P. aeruginosa Molecular clock evaluation dated the emergence of clone ST463 to round 2007.
Genome-wide affiliation evaluation confirmed that 567 genes have been related to clone ST463, together with a number of identified virulence genes associated to the biosynthesis of lipooligosaccharide (LOS) O-antigens and exotoxin. These findings point out that ST463 is increasing with plasmid-borne bla KPC-2 and virulence-related genes in nosocomial infections, and shut surveillance ought to be undertaken sooner or later.
IMPORTANCE Well being care-associated infections, often known as nosocomial infections, are essentially the most frequent hostile occasions in well being care supply worldwide, inflicting excessive charges of morbidity and mortality and excessive well being care prices. Pseudomonas aeruginosa is among the main bacterial species inflicting well being care-associated infections. Carbapenems are the simplest antimicrobial brokers for the remedy of its extreme infections. Nevertheless, the prevalence of carbapenem-resistant P. aeruginosa (CRPA) has been growing quickly in recent times, and our surveillance demonstrated that the prevalence of CRPA reached 38.67% in Zhejiang, China. Genome sequencing of CRPA isolates over a decade confirmed {that a} CRPA clone (ST463) emerged not too long ago.
The clone is extremely proof against β-lactams, together with carbapenems, and fluoroquinolones. Genome-wide affiliation evaluation confirmed that the clone expanded with virulence-related genes and the plasmid-borne carbapenem-resistant gene bla KPC-2 These findings are of great public well being significance, as the data will facilitate the management and minimization of CRPA nosocomial infections.

Full genome sequence of marine Roseobacter lineage member Monaibacterium sp. ALG8 with six plasmids remoted from seawater round brown algae

Monaibacterium sp. ALG8 (=MCCC 1 Okay04733) was remoted from seawater round brown algae. The genome of Monaibacterium sp. ALG8 was sequenced, one round 3,036,380 bp chromosome and 6 round plasmids starting from 12,229 to 151,263 bp have been discovered after meeting. The outcomes of genomic annotation confirmed that Monaibacterium sp. ALG8 lacks the flexibility to degrade alginate, indicating its ecological position might not be immediately associated to the degradation of brown algae.
Emergence and Expansion of a Carbapenem-Resistant Pseudomonas aeruginosa Clone Are Associated with Plasmid-Borne bla KPC-2 and Virulence-Related Genes
The comparability of genomic options within the plasmids confirmed that the majority of those plasmids, besides pALG4, have been horizontally recruited from donors, not ancestors. Based mostly on predicted capabilities, the existence of plasmids might present pressure ALG8 with benefits together with nitrate discount, tolerance of osmotic stress by way of glycine betaine, resistance to heavy steel stress equivalent to mercury and cobalt, degradation of benzoate metabolites equivalent to p-cumate, transformation of the swim-or-stick life-style and enchancment of the immune system with two CRISPR-Cas methods. This examine gives proof for the carbon metabolic patterns of Monaibacterium sp. ALG8 and predicts the capabilities and donors of six plasmids on this pressure, broadening our understanding of the ecological roles of micro organism within the setting round brown algae and the capabilities and evolutionary patterns of plasmids in marine Roseobacter lineage members.

A high-efficiency methodology for site-directed mutagenesis of huge plasmids primarily based on giant DNA fragment amplification and recombinational ligation

Web site-directed mutagenesis for big plasmids is a troublesome job that can’t simply be solved by the standard strategies utilized in many laboratories. On this examine, we developed an efficient methodology for Web site-directed Mutagenesis for Massive Plasmids (SMLP) primarily based on a PCR approach. The SMLP methodology combines a number of efficient approaches, together with a high-efficiency DNA polymerase for the big DNA amplification, two unbiased PCR reactions and a quick recombinational ligation.

pOET-5 Transfer Vector (10ug)
GWB-200106 10 ug Ask for price

pOET3 transfer plasmid
200104 10 µg
EUR 148.18

pOET4 transfer plasmid
200105 10 µg
EUR 148.18

pOET5.1 transfer plasmid
200106 10 µg
EUR 148.18

pOET1.1N_6xHis transfer plasmid
2001011 10 µg
EUR 148.18

pOET1.1C_6xHis transfer plasmid
2001012 10 µg
EUR 148.18

pOET2.1C_6xHis transfer plasmid
2001032 10 µg
EUR 148.18

pOET8.VE2 transfer plasmid
200122 10 µg
EUR 353.43

pOET8.VE3 transfer plasmid
200123 10 µg
EUR 353.43

pOET9 EF1α transfer plasmid
200131 10 µg
EUR 199.33

pOET9 CCAG transfer plasmid
200132 10 µg
EUR 199.33

pOET9 SV40 transfer plasmid
200134 10 µg
EUR 199.33

pOET2.1N/C_6xHis transfer plasmid
2001031 10 µg
EUR 148.18

pOET6 BacMAM transfer plasmid
200107 10 µg
EUR 148.18

pOET 2 N/C_6xHis™ Transfer Vector
GWB-001031 10 ug Ask for price

CCND1 with C-tGFP tag for Nucleus marking (10ug transfection-grade plasmid)
RC100009 10 µg Ask for price

LAMP1 with C-tGFP tag for Lysosome marking (10ug transfection-grade plasmid)
RC100016 10 µg Ask for price

LMNB1 with N-tGFP tag for Nucleus marking (10ug transfection-grade plasmid)
RC100018 10 µg Ask for price

Rab4 with N-tGFP tag for Endosome marking (10ug transfection-grade plasmid)
RC100025 10 µg Ask for price

Rab5 with N-tGFP tag for Endosome marking (10ug transfection-grade plasmid)
RC100026 10 µg Ask for price

RhoB with N-tGFP tag for Endosome marking (10ug transfection-grade plasmid)
RC100027 10 µg Ask for price

CCND1 with C-tRFP tag for Nucleus marking (10ug transfection-grade plasmid)
RC100041 10 µg Ask for price

LAMP1 with C-tRFP tag for Lysosome marking (10ug transfection-grade plasmid)
RC100048 10 µg Ask for price

LMNB1 with N-tRFP tag for Nucleus marking (10ug transfection-grade plasmid)
RC100050 10 µg Ask for price

Rab4 with N-tRFP tag for Endosome marking (10ug transfection-grade plasmid)
RC100057 10 µg Ask for price

Rab5 with N-tRFP tag for Endosome marking (10ug transfection-grade plasmid)
RC100058 10 µg Ask for price

RhoB with N-tRFP tag for Endosome marking (10ug transfection-grade plasmid)
RC100059 10 µg Ask for price

LAMP1 with C-mGFP tag for Lysosome marking (10ug transfection-grade plasmid)
RC100089 10 µg Ask for price

CCND1 with C-mGFP tag for Nucleus marking (10ug transfection-grade plasmid)
RC100094 10 µg Ask for price

LMNB1 with N-mGFP tag for Nucleus marking (10ug transfection-grade plasmid)
RC100095 10 µg Ask for price

GBA2 with C-mGFP tag for Microsome marking (10ug transfection-grade plasmid)
RC100099 10 µg Ask for price

LAMP1 with C-mRFP tag for Lysosome marking (10ug transfection-grade plasmid)
RC100123 10 µg Ask for price

CCND1 with C-mRFP tag for Nucleus marking (10ug transfection-grade plasmid)
RC100128 10 µg Ask for price

LMNB1 with N-mRFP tag for Nucleus marking (10ug transfection-grade plasmid)
RC100129 10 µg Ask for price

GBA2 with C-mRFP tag for Microsome marking (10ug transfection-grade plasmid)
RC100133 10 µg Ask for price

LAMP1 with C-mBFP tag for Lysosome marking (10ug transfection-grade plasmid)
RC100157 10 µg Ask for price

CCND1 with C-mBFP tag for Nucleus marking (10ug transfection-grade plasmid)
RC100162 10 µg Ask for price

LMNB1 with N-mBFP tag for Nucleus marking (10ug transfection-grade plasmid)
RC100163 10 µg Ask for price

GBA2 with C-mBFP tag for Microsome marking (10ug transfection-grade plasmid)
RC100167 10 µg Ask for price

LCK with C-tGFP tag for Plasma memberane marking (10ug transfection-grade plasmid)
RC100017 10 µg Ask for price

LCK with C-tRFP tag for Plasma memberane marking (10ug transfection-grade plasmid)
RC100049 10 µg Ask for price

CLTB with N-tGFP tag for Coated pit marking (10ug transfection-grade plasmid)
RC100010 10 µg Ask for price

CLTB with N-tRFP tag for Coated pit marking (10ug transfection-grade plasmid)
RC100042 10 µg Ask for price

CLTB with N-mGFP tag for Coated Pit marking (10ug transfection-grade plasmid)
RC100071 10 µg Ask for price

CLTB with N-mRFP tag for Coated Pit marking (10ug transfection-grade plasmid)
RC100105 10 µg Ask for price

CLTB with N-mBFP tag for Coated Pit marking (10ug transfection-grade plasmid)
RC100139 10 µg Ask for price

BID with C-tGFP tag for Mitochondria marking (10ug transfection-grade plasmid)
RC100007 10 µg Ask for price

PLK1 with N-tGFP tag for Centrosome marking (10ug transfection-grade plasmid)
RC100023 10 µg Ask for price

PXMP2 with N-tGFP tag for Peroxisome marking (10ug transfection-grade plasmid)
RC100024 10 µg Ask for price

BID with C-tRFP tag for Mitochondria marking (10ug transfection-grade plasmid)
RC100039 10 µg Ask for price

PLK1 with N-tRFP tag for Centrosome marking (10ug transfection-grade plasmid)
RC100055 10 µg Ask for price

PXMP2 with N-tRFP tag for Peroxisome marking (10ug transfection-grade plasmid)
RC100056 10 µg Ask for price

PLK1 with N-mGFP tag for Centrosome marking (10ug transfection-grade plasmid)
RC100070 10 µg Ask for price

BID with C-mGFP tag for Mitochondria marking (10ug transfection-grade plasmid)
RC100090 10 µg Ask for price

PLK1 with N-mRFP tag for Centrosome marking (10ug transfection-grade plasmid)
RC100104 10 µg Ask for price

BID with C-mRFP tag for Mitochondria marking (10ug transfection-grade plasmid)
RC100124 10 µg Ask for price

PLK1 with N-mBFP tag for Centrosome marking (10ug transfection-grade plasmid)
RC100138 10 µg Ask for price

BID with C-mBFP tag for Mitochondria marking (10ug transfection-grade plasmid)
RC100158 10 µg Ask for price

ACTB with N-tGFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)
RC100002 10 µg Ask for price

PDHA1 with C-tGFP tag for Mitochondria marking (10ug transfection-grade plasmid)
RC100006 10 µg Ask for price

PFN1 with N-tGFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)
RC100022 10 µg Ask for price

PDHA1 with C-tRFP tag for Mitochondria marking (10ug transfection-grade plasmid)
RC100038 10 µg Ask for price

PFN1 with N-tRFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)
RC100054 10 µg Ask for price

ATG12 with C-mGFP tag for Auophagasome marking (10ug transfection-grade plasmid)
RC100066 10 µg Ask for price

PDHA1 with C-mGFP tag for Mitochondria marking (10ug transfection-grade plasmid)
RC100091 10 µg Ask for price

ATG12 with C-mRFP tag for Auophagasome marking (10ug transfection-grade plasmid)
RC100100 10 µg Ask for price

PDHA1 with C-mRFP tag for Mitochondria marking (10ug transfection-grade plasmid)
RC100125 10 µg Ask for price

ATG12 with C-mBFP tag for Auophagasome marking (10ug transfection-grade plasmid)
RC100134 10 µg Ask for price

PDHA1 with C-mBFP tag for Mitochondria marking (10ug transfection-grade plasmid)
RC100159 10 µg Ask for price

GAP43 with C-tGFP tag for Neuroal axis marking (10ug transfection-grade plasmid)
RC100013 10 µg Ask for price

GAP43 with C-tRFP tag for Neuroal axis marking (10ug transfection-grade plasmid)
RC100045 10 µg Ask for price

RhoB with N-mGFP tag for Early Endosome marking (10ug transfection-grade plasmid)
RC100075 10 µg Ask for price

Rab4 with N-mGFP tag for Early Endosome marking (10ug transfection-grade plasmid)
RC100077 10 µg Ask for price

Rab5 with N-mGFP tag for Early Endosome marking (10ug transfection-grade plasmid)
RC100078 10 µg Ask for price

RhoB with N-mRFP tag for Early Endosome marking (10ug transfection-grade plasmid)
RC100109 10 µg Ask for price

Rab4 with N-mRFP tag for Early Endosome marking (10ug transfection-grade plasmid)
RC100111 10 µg Ask for price

Rab5 with N-mRFP tag for Early Endosome marking (10ug transfection-grade plasmid)
RC100112 10 µg Ask for price

RhoB with N-mBFP tag for Early Endosome marking (10ug transfection-grade plasmid)
RC100143 10 µg Ask for price

Rab4 with N-mBFP tag for Early Endosome marking (10ug transfection-grade plasmid)
RC100145 10 µg Ask for price

Rab5 with N-mBFP tag for Early Endosome marking (10ug transfection-grade plasmid)
RC100146 10 µg Ask for price

ATG12 with C-tGFP tag for Autophagosome marking (10ug transfection-grade plasmid)
RC100004 10 µg Ask for price

MAPRE3 with C-tGFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)
RC100019 10 µg Ask for price

TUBA1B with N-tGFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)
RC100030 10 µg Ask for price

ATG12 with C-tRFP tag for Autophagosome marking (10ug transfection-grade plasmid)
RC100036 10 µg Ask for price

MAPRE3 with C-tRFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)
RC100051 10 µg Ask for price

MAPRE3 with C-mGFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)
RC100072 10 µg Ask for price

MAPRE3 with C-mRFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)
RC100106 10 µg Ask for price

MAPRE3 with C-mBFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)
RC100140 10 µg Ask for price

SYP with N-tGFP tag for Synaptic vesicles marking (10ug transfection-grade plasmid)
RC100028 10 µg Ask for price

TGOLN with C-tGFP tag for Golgi apparatus marking (10ug transfection-grade plasmid)
RC100029 10 µg Ask for price

SYP with N-tRFP tag for Synaptic vesicles marking (10ug transfection-grade plasmid)
RC100060 10 µg Ask for price

TGOLN with C-tRFP tag for Golgi apparatus marking (10ug transfection-grade plasmid)
RC100061 10 µg Ask for price

TGOLN with C-mGFP tag for Golgi Apparatus marking (10ug transfection-grade plasmid)
RC100088 10 µg Ask for price

PXMP2 (PTS) with N-mGFP tag for Peroxisome marking (10ug transfection-grade plasmid)
RC100096 10 µg Ask for price

TGOLN with C-mRFP tag for Golgi Apparatus marking (10ug transfection-grade plasmid)
RC100122 10 µg Ask for price

PXMP2 (PTS) with N-mRFP tag for Peroxisome marking (10ug transfection-grade plasmid)
RC100130 10 µg Ask for price

TGOLN with C-mBFP tag for Golgi Apparatus marking (10ug transfection-grade plasmid)
RC100156 10 µg Ask for price

PXMP2 (PTS) with N-mBFP tag for Peroxisome marking (10ug transfection-grade plasmid)
RC100164 10 µg Ask for price

MAP1LC3A with N-tGFP tag for Autophagosome marking (10ug transfection-grade plasmid)
RC100020 10 µg Ask for price

MAP1LC3B with N-tGFP tag for Autophagosome marking (10ug transfection-grade plasmid)
RC100021 10 µg Ask for price

MAP1LC3A with N-tRFP tag for Autophagosome marking (10ug transfection-grade plasmid)
RC100052 10 µg Ask for price

MAP1LC3B with N-tRFP tag for Autophagosome marking (10ug transfection-grade plasmid)
RC100053 10 µg Ask for price

MAP1LC3A with N-mGFP tag for Autophagosome marking (10ug transfection-grade plasmid)
RC100068 10 µg Ask for price

MAP1LC3B with N-mGFP tag for Autophagosome marking (10ug transfection-grade plasmid)
RC100069 10 µg Ask for price

MAP1LC3A with N-mRFP tag for Autophagosome marking (10ug transfection-grade plasmid)
RC100102 10 µg Ask for price

MAP1LC3B with N-mRFP tag for Autophagosome marking (10ug transfection-grade plasmid)
RC100103 10 µg Ask for price

MAP1LC3A with N-mBFP tag for Autophagosome marking (10ug transfection-grade plasmid)
RC100136 10 µg Ask for price

MAP1LC3B with N-mBFP tag for Autophagosome marking (10ug transfection-grade plasmid)
RC100137 10 µg Ask for price

B4GalT1 with C-tGFP tag for Golgi apparatus marking (10ug transfection-grade plasmid)
RC100005 10 µg Ask for price

Di-Ras3 with N-tGFP tag for Autophagosome marking (10ug transfection-grade plasmid)
RC100011 10 µg Ask for price

B4GalT1 with C-tRFP tag for Golgi apparatus marking (10ug transfection-grade plasmid)
RC100037 10 µg Ask for price

Di-Ras3 with N-tRFP tag for Autophagosome marking (10ug transfection-grade plasmid)
RC100043 10 µg Ask for price

Di-Ras3 with N-mGFP tag for Autophagosome marking (10ug transfection-grade plasmid)
RC100067 10 µg Ask for price

COX8A (MTS) with C-mGFP tag for Mitochondria marking (10ug transfection-grade plasmid)
RC100092 10 µg Ask for price

Di-Ras3 with N-mRFP tag for Autophagosome marking (10ug transfection-grade plasmid)
RC100101 10 µg Ask for price

COX8A (MTS) with C-mRFP tag for Mitochondria marking (10ug transfection-grade plasmid)
RC100126 10 µg Ask for price

Di-Ras3 with N-mBFP tag for Autophagosome marking (10ug transfection-grade plasmid)
RC100135 10 µg Ask for price

COX8A (MTS) with C-mBFP tag for Mitochondria marking (10ug transfection-grade plasmid)
RC100160 10 µg Ask for price

FAK with N-tGFP tag for Focal adherin fiber marking (10ug transfection-grade plasmid)
RC100012 10 µg Ask for price

VCL with N-tGFP tag for Focal adherin fiber marking (10ug transfection-grade plasmid)
RC100031 10 µg Ask for price

ZYX with C-tGFP tag for Focal adherin fiber marking (10ug transfection-grade plasmid)
RC100032 10 µg Ask for price

FAK with N-tRFP tag for Focal adherin fiber marking (10ug transfection-grade plasmid)
RC100044 10 µg Ask for price

VCL with N-tRFP tag for Focal adherin fiber marking (10ug transfection-grade plasmid)
RC100063 10 µg Ask for price

ZYX with C-tRFP tag for Focal adherin fiber marking (10ug transfection-grade plasmid)
RC100064 10 µg Ask for price

FAK with N-mGFP tag for Focal Adherin Fiber marking (10ug transfection-grade plasmid)
RC100084 10 µg Ask for price

VCL with N-mGFP tag for Focal Adherin Fiber marking (10ug transfection-grade plasmid)
RC100085 10 µg Ask for price

ZYX with C-mGFP tag for Focal Adherin Fiber marking (10ug transfection-grade plasmid)
RC100086 10 µg Ask for price

FAK with N-mRFP tag for Focal Adherin Fiber marking (10ug transfection-grade plasmid)
RC100118 10 µg Ask for price

VCL with N-mRFP tag for Focal Adherin Fiber marking (10ug transfection-grade plasmid)
RC100119 10 µg Ask for price

ZYX with C-mRFP tag for Focal Adherin Fiber marking (10ug transfection-grade plasmid)
RC100120 10 µg Ask for price

FAK with N-mBFP tag for Focal Adherin Fiber marking (10ug transfection-grade plasmid)
RC100152 10 µg Ask for price

VCL with N-mBFP tag for Focal Adherin Fiber marking (10ug transfection-grade plasmid)
RC100153 10 µg Ask for price

ZYX with C-mBFP tag for Focal Adherin Fiber marking (10ug transfection-grade plasmid)
RC100154 10 µg Ask for price

Rab11a with N-mGFP tag for Recycling Endosome marking (10ug transfection-grade plasmid)
RC100082 10 µg Ask for price

Rab11a with N-mRFP tag for Recycling Endosome marking (10ug transfection-grade plasmid)
RC100116 10 µg Ask for price

Rab11a with N-mBFP tag for Recycling Endosome marking (10ug transfection-grade plasmid)
RC100150 10 µg Ask for price

CALR (ETS) with mRFP tag for Endoplasmic Reticulum marking (10ug transfection-grade plasmid)
RC100108 10 µg Ask for price

CALR (ETS) with mBFP tag for Endoplasmic Reticulum marking (10ug transfection-grade plasmid)
RC100142 10 µg Ask for price

B4GalT1 (GTS) with C-mGFP tag for Golgi Apparatus marking (10ug transfection-grade plasmid)
RC100087 10 µg Ask for price

B4GalT1 (GTS) with C-mRFP tag for Golgi Apparatus marking (10ug transfection-grade plasmid)
RC100121 10 µg Ask for price

B4GalT1 (GTS) with C-mBFP tag for Golgi Apparatus marking (10ug transfection-grade plasmid)
RC100155 10 µg Ask for price

pOET Sequencing Primers
GWB-200100 2 x 100 ul Ask for price

Gltp (untagged) - Mouse glycolipid transfer protein (Gltp), (10ug)
MC203852 10 µg Ask for price

Pltp (untagged) - Mouse phospholipid transfer protein (Pltp), (10ug)
MC216773 10 µg Ask for price

Pctp (untagged) - Mouse phosphatidylcholine transfer protein (Pctp), (10ug)
MC209154 10 µg Ask for price

PLTP (Plasma Phospholipid Transfer Protein)
MBS613178-02mL 0.2mL
EUR 650

PLTP (Plasma Phospholipid Transfer Protein)
MBS613178-5x02mL 5x0.2mL
EUR 2765

Gltpd2 (untagged) - Mouse glycolipid transfer protein domain containing 2 (Gltpd2), (10ug)
MC201992 10 µg Ask for price

Ttpa (untagged) - Mouse tocopherol (alpha) transfer protein (Ttpa), (10ug)
MC209849 10 µg Ask for price

Mttp (untagged) - Mouse microsomal triglyceride transfer protein (Mttp), transcript variant 2, (10ug)
MC204263 10 µg Ask for price

Pctp (GFP-tagged) - Mouse phosphatidylcholine transfer protein (Pctp), (10ug)
MG219494 10 µg Ask for price

Ttpa (GFP-tagged) - Mouse tocopherol (alpha) transfer protein (Ttpa), (10ug)
MG221845 10 µg Ask for price

Pitpnm2 (untagged) - Mouse phosphatidylinositol transfer protein, membrane-associated 2 (Pitpnm2), (10ug)
MC224144 10 µg Ask for price

Ttpal (untagged) - Mouse tocopherol (alpha) transfer protein-like (Ttpal), transcript variant 2, (10ug)
MC202535 10 µg Ask for price

Pitpna (untagged) - Mouse phosphatidylinositol transfer protein, alpha (Pitpna), (10ug)
MC206047 10 µg Ask for price

Pitpnb (untagged) - Mouse phosphatidylinositol transfer protein, beta (Pitpnb), (10ug)
MC210037 10 µg Ask for price

PLTP (Plasma Phospholipid Transfer Protein) (Azide Free)
MBS633130-01mg 0.1mg
EUR 630

PLTP (Plasma Phospholipid Transfer Protein) (Azide Free)
MBS633130-5x01mg 5x0.1mg
EUR 2680

Pitpnm2 (GFP-tagged) - Mouse phosphatidylinositol transfer protein membrane-associated 2 (Pitpnm2), (10ug)
MG216492 10 µg Ask for price

Pitpnb (GFP-tagged) - Mouse phosphatidylinositol transfer protein beta (Pitpnb), (10ug)
MG224376 10 µg Ask for price

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (AP)
MBS6431996-01mL 0.1mL
EUR 950

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (AP)
MBS6431996-5x01mL 5x0.1mL
EUR 4120

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (PE)
MBS6432006-01mL 0.1mL
EUR 950

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (PE)
MBS6432006-5x01mL 5x0.1mL
EUR 4120

Pitpnc1 (untagged) - Mouse phosphatidylinositol transfer protein, cytoplasmic 1 (Pitpnc1), (10ug)
MC211221 10 µg Ask for price

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (APC)
MBS6431997-01mL 0.1mL
EUR 950

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (APC)
MBS6431997-5x01mL 5x0.1mL
EUR 4120

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (HRP)
MBS6432000-01mL 0.1mL
EUR 950

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (HRP)
MBS6432000-5x01mL 5x0.1mL
EUR 4120

CETP (untagged) - Human cholesteryl ester transfer protein, plasma (CETP), transcript variant 2
SC336099 10 µg Ask for price

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (FITC)
MBS6431999-01mL 0.1mL
EUR 950

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (FITC)
MBS6431999-5x01mL 5x0.1mL
EUR 4120

Gltpd1 (untagged) - Mouse glycolipid transfer protein domain containing 1 (Gltpd1), (10ug)
MC200085 10 µg Ask for price

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (Biotin)
MBS6431998-01mL 0.1mL
EUR 950

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (Biotin)
MBS6431998-5x01mL 5x0.1mL
EUR 4120

Pitpnm1 (untagged) - Mouse phosphatidylinositol transfer protein, membrane-associated 1 (Pitpnm1), transcript variant 2, (10ug)
MC223145 10 µg Ask for price

PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 750)
MBS6231153-01mL 0.1(mL
EUR 875

PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 750)
MBS6231153-5x01mL 5x0.1mL
EUR 3800

PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 650)
MBS6220478-01mL 0.1(mL
EUR 875

PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 650)
MBS6220478-5x01mL 5x0.1mL
EUR 3800

PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 490)
MBS6199128-01mL 0.1(mL
EUR 875

PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 490)
MBS6199128-5x01mL 5x0.1mL
EUR 3800

PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 550)
MBS6209803-01mL 0.1(mL
EUR 875

PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 550)
MBS6209803-5x01mL 5x0.1mL
EUR 3800

PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 405)
MBS6188453-01mL 0.1(mL
EUR 875

PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 405)
MBS6188453-5x01mL 5x0.1mL
EUR 3800

ExoFectin® Plasmid DNA-into-Exosome Kit (DNA transfection kit for exosome)
P402 20 rxn
EUR 499

Pitpnc1 (GFP-tagged) - Mouse phosphatidylinositol transfer protein cytoplasmic 1 (Pitpnc1), (10ug)
MG224096 10 µg Ask for price

Stard13 (untagged) - Mouse StAR-related lipid transfer (START) domain containing 13 (Stard13), transcript variant 2, (10ug)
MC201577 10 µg Ask for price

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 405)
MBS6432001-01mL 0.1mL
EUR 950

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 405)
MBS6432001-5x01mL 5x0.1mL
EUR 4120

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 490)
MBS6432002-01mL 0.1mL
EUR 950

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 490)
MBS6432002-5x01mL 5x0.1mL
EUR 4120

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 550)
MBS6432003-01mL 0.1mL
EUR 950

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 550)
MBS6432003-5x01mL 5x0.1mL
EUR 4120

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 650)
MBS6432004-01mL 0.1mL
EUR 950

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 650)
MBS6432004-5x01mL 5x0.1mL
EUR 4120

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 750)
MBS6432005-01mL 0.1mL
EUR 950

PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 750)
MBS6432005-5x01mL 5x0.1mL
EUR 4120
Utilizing this methodology, we’ve got achieved quite a lot of mutants for the filamin A gene (7.9 kb) cloned within the pcDNA (5.four kb) or the pLV-U6-CMV-EGFP (9.four kb) plasmids, indicating that this methodology could be utilized to site-directed mutagenesis for the plasmids as much as 17.Three kb. We present that the SMLP methodology has a higher benefit than the standard strategies examined on this examine, and this methodology could be utilized to substitution, deletion, and insertion mutations for each giant and small plasmids in addition to the meeting of three fragments from PCR reactions. Altogether, the SMLP methodology is straightforward, efficient, and useful to the laboratories that require finishing the mutagenesis of huge plasmids.

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