Emergence and Expansion of a Carbapenem-Resistant Pseudomonas aeruginosa Clone Are Associated with Plasmid-Borne bla KPC-2 and Virulence-Related Genes

Emergence and Expansion of a Carbapenem-Resistant Pseudomonas aeruginosa Clone Are Associated with Plasmid-Borne bla KPC-2 and Virulence-Related Genes

beads
Lieven0
Pseudomonas aeruginosa is a serious opportunistic pathogen and one of many main bacterial species inflicting well being care-associated infections. Carbapenems are the simplest antimicrobial brokers for the remedy of extreme infections attributable to P. aeruginosa Nevertheless, our current surveillance demonstrated that the prevalence of carbapenem-resistant P. aeruginosa (CRPA) reached 38.67% in Zhejiang, China.
By analyzing CRPA isolates collected from sufferers from 2006 to 2018, we discovered that 33% of CRPA isolates carried the gene bla KPC-2, which conferred high-level resistance to carbapenems and different β-lactams. Particularly, a CRPA clone, ST463 (sequence sort 463), emerged and has turn out to be the predominant CRPA clone among the many inhabitants. Genome sequencing demonstrated that ST463 enlargement was related to plasmid-borne bla KPC-2 The cellular factor flanking bla KPC-2, the kind IV secretion system, and the profitable enlargement of clone ST463 might need additional favored bla KPC-2 unfold in P. aeruginosa Molecular clock evaluation dated the emergence of clone ST463 to round 2007.
Genome-wide affiliation evaluation confirmed that 567 genes have been related to clone ST463, together with a number of identified virulence genes associated to the biosynthesis of lipooligosaccharide (LOS) O-antigens and exotoxin. These findings point out that ST463 is increasing with plasmid-borne bla KPC-2 and virulence-related genes in nosocomial infections, and shut surveillance ought to be undertaken sooner or later.
IMPORTANCE Well being care-associated infections, often known as nosocomial infections, are essentially the most frequent hostile occasions in well being care supply worldwide, inflicting excessive charges of morbidity and mortality and excessive well being care prices. Pseudomonas aeruginosa is among the main bacterial species inflicting well being care-associated infections. Carbapenems are the simplest antimicrobial brokers for the remedy of its extreme infections. Nevertheless, the prevalence of carbapenem-resistant P. aeruginosa (CRPA) has been growing quickly in recent times, and our surveillance demonstrated that the prevalence of CRPA reached 38.67% in Zhejiang, China. Genome sequencing of CRPA isolates over a decade confirmed {that a} CRPA clone (ST463) emerged not too long ago.
The clone is extremely proof against β-lactams, together with carbapenems, and fluoroquinolones. Genome-wide affiliation evaluation confirmed that the clone expanded with virulence-related genes and the plasmid-borne carbapenem-resistant gene bla KPC-2 These findings are of great public well being significance, as the data will facilitate the management and minimization of CRPA nosocomial infections.

Full genome sequence of marine Roseobacter lineage member Monaibacterium sp. ALG8 with six plasmids remoted from seawater round brown algae

Monaibacterium sp. ALG8 (=MCCC 1 Okay04733) was remoted from seawater round brown algae. The genome of Monaibacterium sp. ALG8 was sequenced, one round 3,036,380 bp chromosome and 6 round plasmids starting from 12,229 to 151,263 bp have been discovered after meeting. The outcomes of genomic annotation confirmed that Monaibacterium sp. ALG8 lacks the flexibility to degrade alginate, indicating its ecological position might not be immediately associated to the degradation of brown algae.
Emergence and Expansion of a Carbapenem-Resistant Pseudomonas aeruginosa Clone Are Associated with Plasmid-Borne bla KPC-2 and Virulence-Related Genes
The comparability of genomic options within the plasmids confirmed that the majority of those plasmids, besides pALG4, have been horizontally recruited from donors, not ancestors. Based mostly on predicted capabilities, the existence of plasmids might present pressure ALG8 with benefits together with nitrate discount, tolerance of osmotic stress by way of glycine betaine, resistance to heavy steel stress equivalent to mercury and cobalt, degradation of benzoate metabolites equivalent to p-cumate, transformation of the swim-or-stick life-style and enchancment of the immune system with two CRISPR-Cas methods. This examine gives proof for the carbon metabolic patterns of Monaibacterium sp. ALG8 and predicts the capabilities and donors of six plasmids on this pressure, broadening our understanding of the ecological roles of micro organism within the setting round brown algae and the capabilities and evolutionary patterns of plasmids in marine Roseobacter lineage members.

A high-efficiency methodology for site-directed mutagenesis of huge plasmids primarily based on giant DNA fragment amplification and recombinational ligation

Web site-directed mutagenesis for big plasmids is a troublesome job that can’t simply be solved by the standard strategies utilized in many laboratories. On this examine, we developed an efficient methodology for Web site-directed Mutagenesis for Massive Plasmids (SMLP) primarily based on a PCR approach. The SMLP methodology combines a number of efficient approaches, together with a high-efficiency DNA polymerase for the big DNA amplification, two unbiased PCR reactions and a quick recombinational ligation.

pLenti-MAGEA3 shRNA-2 Plasmid
PVTBAV13661-2 2 ug
EUR 427.2

pLenti-RUNX3 shRNA-2 Plasmid
PVTBAV20583-2 2 ug
EUR 427.2

pLenti-Slc7a11 shRNA-2 Plasmid
PVTBAV21973-2 2 ug
EUR 427.2

pLenti-STAT3 shRNA-2 Plasmid
PVTBAV22921-2 2 ug
EUR 427.2

pLenti-XRCC5 shRNA-2 Plasmid
PVTBAV26238-2 2 ug
EUR 427.2

SARS-CoV-2 Nucleocapsid Protein, Avi-His-tag
E80027
  • EUR 635.80
  • EUR 4087.60
  • 1 ml
  • 100 ul

Recombinant Porcine IL 2 Protein, Untagged, E.coli-10ug
QP10699-10ug 10ug
EUR 241.2

Recombinant Canine MCP 2 Protein, Untagged, E.coli-10ug
QP10782-10ug 10ug
EUR 241.2

Recombinant HIV-2 Protease Protein, Untagged, E.coli-10ug
QP12271-10ug 10ug
EUR 241.2

Recombinant Human BMP-2 Protein, Untagged, E.coli-10ug
QP5363-10ug 10ug
EUR 284.4

Recombinant SARS-CoV-2 Spike Glycoprotein(S) (D614G), Partial
E80028
  • EUR 388.30
  • EUR 860.20
  • 20 ul
  • 100 ul

SARS-CoV-2 Spike S1 RBD Protein, Avi-His-tag
E80024
  • EUR 635.80
  • EUR 4995.10
  • 100 ul
  • 1 ml

SARS-CoV-2 Spike S1 RBD Protein, Mouse Fc-fusion
E80026
  • EUR 588.50
  • EUR 823.90
  • 20 ul
  • 50 ul

SARS-CoV-2 Spike S1 (16-685) Protein, Avi-His-tag
E80021
  • EUR 635.80
  • EUR 4276.80
  • 100 ul
  • 1 ml

SARS-CoV-2 Spike S1 RBD (V367F) Protein, Avi-His-tag
E80023
  • EUR 635.80
  • EUR 3934.70
  • 100 ul
  • 1 ml

SARS-CoV-2 Spike S1 (13-665) Protein, Fc Fusion, Avi-tag
E80020
  • EUR 635.80
  • EUR 4276.80
  • 100 ul
  • 1 ml

SARS-CoV-2 Spike S1 (16-685) Protein, Fc Fusion, Avi-tag
E80022
  • EUR 635.80
  • EUR 4276.80
  • 100 ul
  • 1 ml

SARS-CoV-2 Spike S1 RBD Protein, Human Fc-Fusion, Avi-Tag
E80025
  • EUR 635.80
  • EUR 3934.70
  • 100 ul
  • 1 ml

Recombinant Human Glutaredoxin-2, GST, E.coli-10ug
QP8154-ec-10ug 10ug
EUR 240

Recombinant Human Arginase-2, GST, E.coli-10ug
QP5676-ec-10ug 10ug
EUR 240

Recombinant Human CXCL2/ MIP-2 Protein, Untagged, E.coli-10ug
QP1016-10ug 10ug
EUR 284.4

Recombinant Bovine FGF 2 Protein, Untagged, Native Protein-10ug
QP10599-10ug 10ug
EUR 426

Recombinant Human MMP-2 Protein, His, HEK 293-10ug
QP10790-10ug 10ug
EUR 241.2

Recombinant Influenza Parainfluenza Type-2 Protein, Untagged, E.coli-10ug
QP12960-10ug 10ug
EUR 186

Recombinant Human STC 2 Protein, Untagged, HEK 293-10ug
QP13613-10ug 10ug
EUR 241.2

Recombinant Mouse MCP-2/ CCL8 Protein, Untagged, E.coli-10ug
QP5471-10ug 10ug
EUR 284.4

Recombinant Bovine FGF 2 Protein, Untagged, E.coli-10ug
QP10599-EC-10ug 10ug
EUR 186

Recombinant Human Metaxin-2 Protein, GST, E.coli-10ug
QP6393-ec-10ug 10ug
EUR 326.4

Recombinant Mouse Thrombospondin-2 Protein, His, E.coli-10ug
QP6786-ec-10ug 10ug
EUR 326.4

Recombinant Mouse Thrombospondin-2 Protein, His, Yeast-10ug
QP6786-ye-10ug 10ug
EUR 326.4

Recombinant E.coli Thioredoxin-2 Protein, His, Yeast-10ug
QP7012-ye-10ug 10ug
EUR 434.4

Recombinant Human Prohibitin-2 Protein, GST, E.coli-10ug
QP8027-ec-10ug 10ug
EUR 240

Recombinant Human Talin-2 Protein, His, Yeast-10ug
QP8291-ye-10ug 10ug
EUR 283.2

Recombinant Mouse Bcl-2 Protein, His, E.coli-10ug
QP5710-ec-10ug 10ug
EUR 186

Recombinant Human Plastin-2 Protein, His, Yeast-10ug
QP6289-ye-10ug 10ug
EUR 283.2

Recombinant Human Galectin-2 Protein, GST, E.coli-10ug
QP6295-ec-10ug 10ug
EUR 240

Recombinant Human Twinfilin-2 Protein, GST, E.coli-10ug
QP7777-ec-10ug 10ug
EUR 240

Recombinant Chicken Vitellogenin-2 Protein, His, E.coli-10ug
QP8859-ec-10ug 10ug
EUR 391.2

Recombinant Mouse Renin-2 Protein, His, E.coli-10ug
QP8886-ec-10ug 10ug
EUR 326.4

Recombinant Human Metallothionein-2 Protein, GST, E.coli-10ug
QP8894-ec-10ug 10ug
EUR 240

Recombinant Human Semenogelin-2 Protein, His, Yeast-10ug
QP9386-ye-10ug 10ug
EUR 369.6

Recombinant Human Hyaluronidase-2 Protein, His, Yeast-10ug
QP9753-ye-10ug 10ug
EUR 283.2

Recombinant Human Ficolin-2 Protein, His, Yeast-10ug
QP9759-ye-10ug 10ug
EUR 283.2

Recombinant Zebrafish Metallothionein-2 Protein, His, Yeast-10ug
QP9826-ye-10ug 10ug
EUR 434.4

Recombinant Mouse Arginase-2, His-SUMO, E.coli-10ug
QP5677-ec-10ug 10ug
EUR 326.4

Recombinant Dengue Virus Dengue Envelope-2 Protein, Untagged, Insect-10ug
QP11626-10ug 10ug
EUR 241.2

Recombinant Human IGF-2/ IGF-II Protein, Untagged, E.coli-10ug
QP5264-10ug 10ug
EUR 163.2

Recombinant Transmembrane Protease Serine 2 Protein, Partial
E80016
  • EUR 470.80
  • EUR 753.50
  • EUR 1824.90
  • 20 ul
  • 100 ul
  • 500 ul

Recombinant Human CCL24/ Eotaxin-2/ MPIF-2 Protein, His, E.coli-10ug
QP8522-ec-10ug 10ug
EUR 240

KRTAP3-2 cloning plasmid
CSB-CL871617HU-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the KRTAP3-2 gene.

KRTAP9-2 cloning plasmid
CSB-CL880139HU1-10ug 10ug
EUR 308.4
Description: A cloning plasmid for the KRTAP9-2 gene.

KRTAP9-2 cloning plasmid
CSB-CL880139HU2-10ug 10ug
EUR 308.4
Description: A cloning plasmid for the KRTAP9-2 gene.

KRTAP12-2 cloning plasmid
CSB-CL012606HU-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the KRTAP12-2 gene.

KRTAP4-2 cloning plasmid
CSB-CL861178HU-10ug 10ug
EUR 279.6
Description: A cloning plasmid for the KRTAP4-2 gene.

Recombinant Human Bcl 2 (minus BH4 domain) Protein, His, E.coli-10ug
QP11138-10ug 10ug
EUR 241.2

Recombinant Human 2-5A-dependent ribonuclease Protein, His-SUMO, E.coli-10ug
QP6997-10ug 10ug
EUR 186

Recombinant Human SerpinB2/ PAI-2 Protein, Untagged, E.coli-10ug
QP10161-ec-10ug 10ug
EUR 348

Recombinant Human TIMP2/ TIMP-2 Protein, Untagged, E.coli-10ug
QP10162-ec-10ug 10ug
EUR 348

Recombinant Human CCL8/ MCP-2 Protein, Untagged, E.coli-10ug
QP10231-ec-10ug 10ug
EUR 348

Recombinant Human IL2/ Interleukin-2 Protein, Untagged, E.coli-10ug
QP10309-ec-10ug 10ug
EUR 184.8

Recombinant Human IGF1 Isoform 2 Protein, Untagged, E.coli-10ug
QP10390-ec-10ug 10ug
EUR 184.8

Recombinant Human CCL8/ MCP-2 Protein, GST, E.coli-10ug
QP4994-ec-10ug 10ug
EUR 240

Recombinant Human 2-oxoglutarate dehydrogenase, His-SUMO, E.coli-10ug
QP6447-ec-10ug 10ug
EUR 240

Recombinant Rat Neuroendocrine convertase 2 Protein, His, E.coli-10ug
QP6473-ec-10ug 10ug
EUR 391.2

Recombinant Rat SerpinB2/ PAI-2 Protein, His, Yeast-10ug
QP6669-ye-10ug 10ug
EUR 326.4

Recombinant Human Tryptase beta-2 Protein, His, E.coli-10ug
QP6832-ec-10ug 10ug
EUR 240

Recombinant Mouse Tryptase beta-2 Protein, GST, E.coli-10ug
QP6833-ec-10ug 10ug
EUR 326.4

Recombinant E.coli Thioredoxin-2 Protein, His-SUMO, E.coli-10ug
QP7012-ec-10ug 10ug
EUR 391.2

Recombinant Macaque CXCL10/ Crg-2 Protein, His, E.coli-10ug
QP7849-ec-10ug 10ug
EUR 391.2

Recombinant Human Thioredoxin-2/ TXN2 Protein, GST, E.coli-10ug
QP8009-ec-10ug 10ug
EUR 240

Recombinant Human Calponin-2 Protein, His-SUMO, E.coli-10ug
QP8036-ec-10ug 10ug
EUR 240

Recombinant Human Thioredoxin reductase 2, His-SUMO, E.coli-10ug
QP8200-ec-10ug 10ug
EUR 240

Recombinant Human Talin-2 Protein, His-SUMO, E.coli-10ug
QP8291-ec-10ug 10ug
EUR 240

Recombinant Human Angiopoietin-2/ ANG2 Protein, His, E.coli-10ug
QP8512-ec-10ug 10ug
EUR 240

Recombinant Human TIMP2/ TIMP-2 Protein, GST, E.coli-10ug
QP8571-ec-10ug 10ug
EUR 240

Recombinant Mouse CXCL2/ MIP-2 Protein, His, E.coli-10ug
QP8651-ec-10ug 10ug
EUR 326.4

Recombinant Human Glutamate carboxypeptidase 2 Protein, His, E.coli-10ug
QP8726-ec-10ug 10ug
EUR 240

Recombinant Human Caspase-2 Protein, His-SUMO, E.coli-10ug
QP5762-ec-10ug 10ug
EUR 240

Recombinant Mouse Desmoglein-2 Protein, His-SUMO, E.coli-10ug
QP5947-ec-10ug 10ug
EUR 391.2

Recombinant Human Elongation factor 2 Protein, His, E.coli-10ug
QP5962-ec-10ug 10ug
EUR 240

Recombinant Human Estrogen Receptor 2 Protein, His, Yeast-10ug
QP6000-ye-10ug 10ug
EUR 283.2

Recombinant Mouse Hyaluronan synthase 2 Protein, His, E.coli-10ug
QP6144-ec-10ug 10ug
EUR 326.4

Recombinant Mouse Hyaluronan synthase 2 Protein, His, Yeast-10ug
QP6144-ye-10ug 10ug
EUR 326.4

Recombinant Human IL2/ Interleukin-2 Protein, GST, E.coli-10ug
QP6216-ec-10ug 10ug
EUR 240

Recombinant Human IL2/ Interleukin-2 Protein, His, Yeast-10ug
QP6216-ye-10ug 10ug
EUR 283.2

Recombinant Rabbit IL2/ Interleukin-2 Protein, His, E.coli-10ug
QP6218-ec-10ug 10ug
EUR 391.2

Recombinant Human Integrin alpha-2 Protein, His, Yeast-10ug
QP6238-ye-10ug 10ug
EUR 283.2

Recombinant Human Plastin-2 Protein, His-SUMO, E.coli-10ug
QP6289-ec-10ug 10ug
EUR 240

Recombinant Human Septin-2 Protein, His-SUMO, E.coli-10ug
QP7552-ec-10ug 10ug
EUR 326.4

Recombinant Human Clavesin-2 Protein, His-SUMO, E.coli-10ug
QP7684-ec-10ug 10ug
EUR 240

Recombinant Mouse 2-oxoglutarate dehydrogenase, His-SUMO, E.coli-10ug
QP7717-ec-10ug 10ug
EUR 326.4

Recombinant Human Sesquipedalian-2 Protein, His-SUMO, E.coli-10ug
QP7760-ec-10ug 10ug
EUR 240

Recombinant Human Complexin-2/ CPLX2 Protein, GST, E.coli-10ug
QP7773-ec-10ug 10ug
EUR 326.4

Recombinant Human Vasohibin-2 Protein, His-SUMO, E.coli-10ug
QP7784-ec-10ug 10ug
EUR 326.4

Recombinant E.coli GTP cyclohydrolase-2 Protein, His, E.coli-10ug
QP8879-ec-10ug 10ug
EUR 391.2

Recombinant Mouse Angiopoietin-2/ ANG2 Protein, His, E.coli-10ug
QP8884-ec-10ug 10ug
EUR 326.4

Recombinant Human Retinal dehydrogenase 2 Protein, His, Yeast-10ug
QP8968-ye-10ug 10ug
EUR 369.6

Recombinant Mouse Elongation factor 2 Protein, His, Yeast-10ug
QP9112-ye-10ug 10ug
EUR 326.4

Recombinant Human MBL-2/ MBL Protein, His, Yeast-10ug
QP9268-ye-10ug 10ug
EUR 283.2

Recombinant Bovine Serpin A3-2 Protein, His, Yeast-10ug
QP9699-ye-10ug 10ug
EUR 434.4

Recombinant Human CCL8/ MCP-2 Protein, His, Yeast-10ug
QP4994-ye-10ug 10ug
EUR 283.2

Recombinant Human BMP-2 Protein, Untagged, HEK 293-10ug
QP5363-HEK-10ug 10ug
EUR 261.6

Recombinant Human FKBP6 Protein, His, -10ug
QP10610-10ug 10ug
EUR 241.2

Recombinant Human NAP-2/ PPBP/ CXCL7 Protein, Untagged, E.coli-10ug
QP10212-ec-10ug 10ug
EUR 348

Recombinant Rat NAP-2/ PPBP/ CXCL7 Protein, Untagged, E.coli-10ug
QP10285-ec-10ug 10ug
EUR 348

Recombinant Human Ribonucleoprotein PTB-binding 2 Protein, His, Yeast-10ug
QP9902-ye-10ug 10ug
EUR 283.2

Recombinant Human Growth/ differentiation factor 2 Protein, His, Yeast-10ug
QP9936-ye-10ug 10ug
EUR 283.2

Recombinant Human Exportin-2 Protein, His-SUMO, Invitro-E.coli-10ug
QP9974-iv-10ug 10ug
EUR 936

Recombinant Rat MMP-2/ CLG4A Protein Protein, His, Yeast-10ug
QP6369-ye-10ug 10ug
EUR 326.4

Recombinant Human Nanos homolog 2 Protein, His-SUMO, E.coli-10ug
QP6402-ec-10ug 10ug
EUR 240

Recombinant Human Nuclear transport factor 2 Protein, GST, E.coli-10ug
QP6442-ec-10ug 10ug
EUR 240

Recombinant Human Phosphomannomutase 2/ PMM2/ CDG1 Protein, GST, E.coli-10ug
QP6508-ec-10ug 10ug
EUR 240

Recombinant Human PTGS2/ COX2/ PGHS-2 Protein, His, E.coli-10ug
QP6551-ec-10ug 10ug
EUR 240

Recombinant Rat SerpinB2/ PAI-2 Protein, His-SUMO, E.coli-10ug
QP6669-ec-10ug 10ug
EUR 326.4

Recombinant Human Serine palmitoyltransferase 2 Protein, His-SUMO, E.coli-10ug
QP6726-ec-10ug 10ug
EUR 240

Recombinant Human Tumor suppressor candidate 2 Protein, GST, E.coli-10ug
QP6856-ec-10ug 10ug
EUR 240

Recombinant Human CD112/ Nectin-2/ PVRL2 Protein, His, E.coli-10ug
QP7863-ec-10ug 10ug
EUR 240

Recombinant Human GMP reductase 2 Protein, His-SUMO, E.coli-10ug
QP8126-ec-10ug 10ug
EUR 240

Recombinant Human AGO2/ Argonaute 2/ EIF2C2 Protein, His, E.coli-10ug
QP8243-ec-10ug 10ug
EUR 240

Recombinant Human AGO2/ Argonaute 2/ EIF2C2 Protein, His, Yeast-10ug
QP8243-ye-10ug 10ug
EUR 283.2

Recombinant Human RuvB-like 2 Protein, His-SUMO, E.coli-10ug
QP8295-ec-10ug 10ug
EUR 326.4

Recombinant Human Glucosidase 2 subunit beta Protein, GST, E.coli-10ug
QP8431-ec-10ug 10ug
EUR 240

Recombinant Human IGF-2/ IGF-II Protein, GST, E.coli-10ug
QP8601-ec-10ug 10ug
EUR 240

Recombinant Human NAP-2/ PPBP/ CXCL7 Protein, GST, E.coli-10ug
QP8641-ec-10ug 10ug
EUR 240

Recombinant Human Laminin subunit beta-2 Protein, His, E.coli-10ug
QP8788-ec-10ug 10ug
EUR 240

Recombinant Human CALCB/ CGPR/ Calcitonin 2 Protein, GST, E.coli-10ug
QP5747-ec-10ug 10ug
EUR 240

Recombinant Human Calpain-2 catalytic subunit Protein, His, E.coli-10ug
QP5756-ec-10ug 10ug
EUR 240

Recombinant Human CETN2/ Centrin 2 Protein, His-SUMO, E.coli-10ug
QP5820-ec-10ug 10ug
EUR 240

Recombinant Human CFL2/ cofilin 2/ ADF Protein, GST, E.coli-10ug
QP5825-ec-10ug 10ug
EUR 240

Recombinant Human Clathrin heavy chain 2 Protein, His, E.coli-10ug
QP5842-ec-10ug 10ug
EUR 240

Recombinant Human Clathrin heavy chain 2 Protein, His, Yeast-10ug
QP5842-ye-10ug 10ug
EUR 283.2

Recombinant Human Egl nine homolog 2 Protein, His, E.coli-10ug
QP5968-ec-10ug 10ug
EUR 240

Recombinant Human Glycerol kinase 2 Protein, His-SUMO, E.coli-10ug
QP6085-ec-10ug 10ug
EUR 240

Recombinant Macaque IL2/ Interleukin-2 Protein, His-SUMO, E.coli-10ug
QP6217-ec-10ug 10ug
EUR 391.2

Recombinant Human Integrin alpha-2 Protein, His-SUMO, E.coli-10ug
QP6238-ec-10ug 10ug
EUR 240

Recombinant Human JAM-2/ JAM-B Protein, His, E.coli-10ug
QP6247-ec-10ug 10ug
EUR 240

Recombinant Human JAM-2/ JAM-B Protein, His, Yeast-10ug
QP6247-ye-10ug 10ug
EUR 283.2

Recombinant Mouse Hemoglobin subunit beta-2 Protein, GST, E.coli-10ug
QP7346-ec-10ug 10ug
EUR 326.4

Recombinant Mouse Hemoglobin subunit beta-2 Protein, His, Yeast-10ug
QP7346-ye-10ug 10ug
EUR 326.4

Recombinant Human Protein delta homolog 2 Protein, GST, E.coli-10ug
QP7755-ec-10ug 10ug
EUR 240

Recombinant Human Protein delta homolog 2 Protein, His, Yeast-10ug
QP7755-ye-10ug 10ug
EUR 283.2

Recombinant Rat BDNF Protein Isoform 2 Protein, His, E.coli-10ug
QP8869-ec-10ug 10ug
EUR 326.4

Recombinant Human Orexin receptor type 2 Protein, His, Yeast-10ug
QP9175-ye-10ug 10ug
EUR 369.6

Recombinant Human Neuropeptide FF receptor 2 Protein, His, Yeast-10ug
QP9310-ye-10ug 10ug
EUR 283.2

Recombinant Human Transmembrane protease serine 2 Protein, His, Yeast-10ug
QP9439-ye-10ug 10ug
EUR 326.4

Recombinant Mouse Protein delta homolog 2 Protein, His, Yeast-10ug
QP9838-ye-10ug 10ug
EUR 369.6

Recombinant Human IL12RB2/ IL12R-beta 2 Protein, His, Yeast-10ug
QP9881-ye-10ug 10ug
EUR 283.2

Recombinant Human AK2/ Adenylate kinase 2 Protein, GST, E.coli-10ug
QP5635-ec-10ug 10ug
EUR 240

pDONR223-CD73 Plasmid
PVTB00480-2 2 ug
EUR 427.2

Recombinant Human Actin-related protein 2/ 3 complex subunit 2 Protein, GST, E.coli-10ug
QP8442-ec-10ug 10ug
EUR 240

Recombinant Human Tryptophan 5-hydroxylase 2 Protein, His, Invitro-E.coli-10ug
QP10004-iv-10ug 10ug
EUR 554.4

Recombinant Human Bcl 2 (minus BH1 domain) Protein, His, E.coli-10ug
QP10491-HIS-10ug 10ug
EUR 241.2

Recombinant Human Bcl 2 (minus BH2 domain) Protein, His, E.coli-10ug
QP10492-HIS-10ug 10ug
EUR 241.2

Recombinant Human Bcl 2 (minus BH3 domain) Protein, His, E.coli-10ug
QP10493-HIS-10ug 10ug
EUR 241.2

Recombinant Human Bcl 2 (minus NWGR domain) Protein, His, E.coli-10ug
QP10494-HIS-10ug 10ug
EUR 241.2

Recombinant Dog Trypsin 2/ PRSS2 Protein, His-SUMO, Invitro-E.coli-10ug
QP9996-iv-10ug 10ug
EUR 936

Recombinant Rat MMP-2/ CLG4A Protein Protein, His-SUMO, E.coli-10ug
QP6369-ec-10ug 10ug
EUR 326.4

Recombinant Human Myelin expression factor 2 Protein, His-SUMO, E.coli-10ug
QP6399-ec-10ug 10ug
EUR 240

Recombinant Human Arylamine N-acetyltransferase 2 Protein, His-SUMO, E.coli-10ug
QP6405-ec-10ug 10ug
EUR 326.4

Recombinant Human Parathyroid hormone 2 receptor Protein, His-SUMO, E.coli-10ug
QP6552-ec-10ug 10ug
EUR 240

Recombinant Mouse Serum amyloid A-2 protein Protein, His, E.coli-10ug
QP6649-ec-10ug 10ug
EUR 326.4

Recombinant Mouse Excitatory amino acid transporter 2 Protein, GST, E.coli-10ug
QP6691-ec-10ug 10ug
EUR 326.4

Recombinant Mouse Excitatory amino acid transporter 2 Protein, GST, Yeast-10ug
QP6691-ye-10ug 10ug
EUR 326.4

Recombinant Human Suppressor of cytokine signaling 2 Protein, GST, E.coli-10ug
QP6712-ec-10ug 10ug
EUR 326.4

Recombinant Human Transcription factor SOX-2 Protein, His-SUMO, E.coli-10ug
QP6718-ec-10ug 10ug
EUR 240

Recombinant Human Protein-tyrosine sulfotransferase 2 Protein, His-SUMO, E.coli-10ug
QP6834-ec-10ug 10ug
EUR 240

Recombinant S. cerevisiae Glycerol 2-dehydrogenase (NADP(+)) Protein, His, Yeast-10ug
QP7055-ye-10ug 10ug
EUR 434.4

Recombinant Mouse Secretoglobin family 3A member 2 Protein, His, Yeast-10ug
QP7929-ye-10ug 10ug
EUR 369.6

Recombinant Human Calcineurin subunit B type 2 Protein, GST, E.coli-10ug
QP8006-ec-10ug 10ug
EUR 240

Recombinant Human Ribonucleoprotein PTB-binding 2 Protein, His-SUMO, E.coli-10ug
QP8116-ec-10ug 10ug
EUR 240

Recombinant Human Transcription factor 7-like 2 Protein, His, E.coli-10ug
QP8221-ec-10ug 10ug
EUR 326.4

Recombinant Human ANGPTL2/ Angiopoietin-like 2 Protein, His-SUMO, E.coli-10ug
QP8238-ec-10ug 10ug
EUR 240

Recombinant Human Protein-arginine deiminase type-2 Protein, His, Yeast-10ug
QP8266-ye-10ug 10ug
EUR 283.2

Recombinant Human Interleukin enhancer-binding factor 2 Protein, GST, E.coli-10ug
QP8309-ec-10ug 10ug
EUR 240

Recombinant Human Inosine-5'-monophosphate dehydrogenase 2 Protein, GST, E.coli-10ug
QP8353-ec-10ug 10ug
EUR 240

Recombinant Human Proteasome subunit beta type-2 Protein, GST, E.coli-10ug
QP8364-ec-10ug 10ug
EUR 240

Recombinant Human Paired box protein Pax-2 Protein, GST, E.coli-10ug
QP8719-ec-10ug 10ug
EUR 240

Recombinant Human Collagen alpha-2(IV) chain Protein, His, E.coli-10ug
QP8734-ec-10ug 10ug
EUR 240

Recombinant Human Bcl-2-like protein 11 Protein, His, Yeast-10ug
QP5712-ye-10ug 10ug
EUR 283.2

Recombinant Pig Calpain-2 catalytic subunit Protein, His-SUMO, E.coli-10ug
QP5757-ec-10ug 10ug
EUR 391.2

Recombinant Human Cancer/ testis antigen 2 Protein, His-SUMO, E.coli-10ug
QP5883-ec-10ug 10ug
EUR 240

Recombinant Human Bifunctional epoxide hydrolase 2 Protein, His-SUMO, E.coli-10ug
QP5986-ec-10ug 10ug
EUR 240

Recombinant Human Eyes absent homolog 2 Protein, His-SUMO, E.coli-10ug
QP6005-ec-10ug 10ug
EUR 240

Recombinant Human Fatty acid desaturase 2 Protein, His-SUMO, E.coli-10ug
QP6016-ec-10ug 10ug
EUR 326.4

Recombinant Human Fos-related antigen 2 Protein, His-SUMO, E.coli-10ug
QP6053-ec-10ug 10ug
EUR 240

Recombinant Mouse LOXL2/ Lysyl oxidase homolog 2 Protein, His, E.coli-10ug
QP6317-ec-10ug 10ug
EUR 326.4

Recombinant Mouse LOXL2/ Lysyl oxidase homolog 2 Protein, His, Yeast-10ug
QP6317-ye-10ug 10ug
EUR 326.4

Recombinant Human Heat shock protein beta-2 Protein, GST, E.coli-10ug
QP7544-ec-10ug 10ug
EUR 240

Recombinant Human Oligodendrocyte transcription factor 2 Protein, His-SUMO, E.coli-10ug
QP7608-ec-10ug 10ug
EUR 240

Recombinant Human Bcl-2-like protein 15 Protein, GST, E.coli-10ug
QP7698-ec-10ug 10ug
EUR 326.4

Recombinant Rat A2M/ CPAMD5/ Alpha-2-macroglobulin Protein, His, Yeast-10ug
QP8951-ye-10ug 10ug
EUR 326.4

Recombinant Human Peroxisomal multifunctional enzyme type 2 Protein, His, Yeast-10ug
QP9186-ye-10ug 10ug
EUR 283.2

Recombinant Human Butyrophilin subfamily 2 member A1 Protein, His, Yeast-10ug
QP9818-ye-10ug 10ug
EUR 283.2

Recombinant Rat Type-2 angiotensin II receptor Protein, GST, E.coli-10ug
QP5626-ec-10ug 10ug
EUR 326.4

Recombinant Rat Type-2 angiotensin II receptor Protein, His, Yeast-10ug
QP5626-ye-10ug 10ug
EUR 326.4

Recombinant Human AP-2 complex subunit mu Protein, His, Yeast-10ug
QP5658-ye-10ug 10ug
EUR 369.6

Recombinant Mouse PRDX2 Protein, His, E.coli-10ug
QP10500-10ug 10ug
EUR 241.2

Recombinant Human TGFBR3 Protein, GST, E.coli-10ug
QP10501-10ug 10ug
EUR 675.6

Recombinant Human ACP5 Protein, Untagged, Insect-10ug
QP10502-10ug 10ug
EUR 241.2
Utilizing this methodology, we’ve got achieved quite a lot of mutants for the filamin A gene (7.9 kb) cloned within the pcDNA (5.four kb) or the pLV-U6-CMV-EGFP (9.four kb) plasmids, indicating that this methodology could be utilized to site-directed mutagenesis for the plasmids as much as 17.Three kb. We present that the SMLP methodology has a higher benefit than the standard strategies examined on this examine, and this methodology could be utilized to substitution, deletion, and insertion mutations for each giant and small plasmids in addition to the meeting of three fragments from PCR reactions. Altogether, the SMLP methodology is straightforward, efficient, and useful to the laboratories that require finishing the mutagenesis of huge plasmids.

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