Two Plasmid-Based Systems for CRISPR/Cas9 Mediated Knockout of Target Genes

Two Plasmid-Based Systems for CRISPR/Cas9 Mediated Knockout of Target Genes

CRISPR/Cas9-based gene modifying is a current advance that enables for the knockout or alteration of goal genes inside mammalian cells. Many variations of the method exist, however right here we describe two programs of plasmid-based CRISPR gene knockout which collectively permit for the selective knockout of nearly any gene goal. In contrast with different CRISPR-based […]

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Gentaur Plasmids

6His-MBP-TEV-huAsCpf1 6His-MBP-TEV-huLbCpf1 AAVS1 T2 CRIPR in pX330 Acinetobacter G-CaMP3 IFN-Beta_pGL3 IKKalpha (S176ES180E)-pcw107-V5 pAd/BLOCK-iT-DEST RNAi Gateway Vector pBluescriptR-ABCB4 pCAG-T7pol pCAMBIA1300-mCherry pCDH-CMV-exosome booster-EF1-copGFP-Puro vector pCDH-CMV-exosome booster-EF1-Hygro pCDH-CMV-mRFP1-EGFP-LC3B-EF1-Puro pCDNA3.1- NLS- NgAgo Plasmid pCDNA3.1-FURIN pCDNA3.1-SLC28A1-FLAG pCDNA3-Flag mTOR pCHO1.0 pCMV3-HA-ULK1 pCMV-Myc-ABCB4 pCS2-MET pECMV-3-FLAG-DNMT1 pECMV-3-FLAG-GUCY2C pECMV-Brca1-m-myc-FLAG pEE12.4 Plasmid pEE14.4 vector pEE6.4 Plasmid pET- MthRnl pGR107 pHIS1525 piggyBac-133CAR_opt-puro PiggyBac-hAsCpf1 pIZ-V5-His pK18mobSacB-xa2 Plasmid […]

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