The human intestine microbiome contains helpful, commensal and pathogenic micro organism that possess antimicrobial resistance (AMR) genes and change these predominantly by means of conjugative plasmids. Escherichia coli is a major factor of the gastrointestinal microbiome and is often non-pathogenic on this area of interest. In distinction, extra-intestinal pathogenic E. coli (ExPEC) together with ST131 might occupy different environments just like the urinary tract or bloodstream the place they specific genes enabling AMR and host cell adhesion like kind 1 fimbriae.
The extent to which commensal E. coli and uropathogenic ExPEC ST131 share AMR genes stays understudied at a genomic degree, and we examined this right here utilizing a preterm toddler resistome. We discovered that particular person ST131 had small variations in AMR gene content material relative to a bigger shared resistome. Comparisons with a variety of plasmids frequent in ST131 confirmed that AMR gene composition was pushed by conjugation, recombination and cellular genetic parts.
Plasmid pEK499 had prolonged areas in most ST131 Clade C isolates, and it had proof of a co-evolutionary sign based mostly on protein-level interactions with chromosomal gene merchandise, as did pEK204 that had a kind IV fimbrial pil operon. ST131 possessed intensive range of selective kind 1, kind IV, P and F17-like fimbriae genes that was highest in subclade C2. The construction and composition of AMR genes, plasmids and fimbriae range extensively in ST131 Clade C and this will likely mediate pathogenicity and an infection outcomes.
Growth of a simplified and cheap RNA depletion methodology for plasmid DNA purification utilizing measurement choice magnetic beads (SSMBs)
Plasmid DNA (pDNA) isolation from bacterial cells is likely one of the most typical and significant steps in molecular cloning and biomedical analysis. Nearly all pDNA purification includes disruption of micro organism, removing of membrane lipids, proteins and genomic DNA, purification of pDNA from bulk lysate, and focus of pDNA for downstream purposes.
Whereas many liquid-phase and solid-phase pDNA purification strategies are used, the ultimate pDNA preparations are often contaminated with diverse levels of host RNA, which can’t be fully digested by RNase A. To develop a easy, cost-effective, and but efficient methodology for RNA depletion, we investigated whether or not commercially accessible measurement choice magnetic beads (SSMBs), corresponding to Magazine-Bind® TotalPure NGS Equipment (or Magazine-Bind), can fully deplete bacterial RNA in pDNA preparations.
On this proof-of-principle research, we demonstrated that, in contrast with RNase A digestion and two business plasmid affinity purification kits, the SSMB methodology was extremely environment friendly in depleting contaminating RNA from pDNA minipreps. Gene transfection and bacterial colony formation assays revealed that pDNA purified from SSMB methodology had superior high quality and integrity to pDNA samples cleaned up by RNase A digestion and/or business plasmid purification kits.
We additional demonstrated that the SSMB methodology fully depleted contaminating RNA in large-scale pDNA samples. Moreover, the Magazine-bind-based SSMB methodology prices solely 5-10% of most business plasmid purification kits on a per pattern foundation. Thus, the reported SSMB methodology generally is a useful and cheap software for the removing of bacterial RNA for routine pDNA preparations.
Emergence of plasmid-mediated tigecycline resistance tet(X4) gene in Escherichia coli remoted from poultry, meals and the surroundings in South Asia
The latest emergence of mobile-tigecycline resistance tet(X) genes in human and animals in China critically threats the medical utility of tigecycline. Right here we targeted on the isolation and molecular characterization of plasmid-mediated tigecycline resistance tet(X4)-positive E. coli from completely different sources in Pakistan utilizing MinION and Illumina sequencing.
The tet(X4) gene was detected in 4 E. coli isolates from poultry, rooster meat, wild fowl and the slaughterhouse wastewater in Pakistan. Co-existence of colistin resistance mcr-1 gene was additionally detected in three isolates. The 4 isolates belonged to completely different sequence sorts and the tet(X4) gene was positioned on plasmids starting from 12,331 bp to 113,610 bp belonging to IncFII and IncQ replicon sorts with two genetic contexts ISCR2-tet(X4)-abh-ISCR2-lysR-floR-virD2 and ΔISCR2-abh-tet(X4)-ISCR2-virD2-floR, respectively.
In all of the 4 E. coli strains, tet(X4) was transferable by conjugation to E. coli J53 host pressure. As well as, three of 4 strains transferred tet(X4) to a wild-type carbapenem resistant E. coli pressure. To our information, that is the primary report of the emergence of plasmid-mediated tet(X4) gene from Pakistan. The convergence of tigecycline and colistin resistance in South Asia is a critical risk to human well being.
A local conjugative plasmid confers potential selective benefits to plant growth-promoting Bacillus velezensis pressure GH1-13
The conjugative plasmid (pBV71) probably confers a selective benefit to Bacillus velezensis pressure GH1-13, though a selective marker gene is but to be recognized. Right here we present that few non-mucoid wild-type GH1-13 cells are spontaneously transformed to mucoid variants with or with out the lack of pBV71. Mucoid phenotypes, which include or lack the plasmid, grow to be delicate to bacitracin, gramicidin, selenite, and tellurite.
Utilizing the variations in antibiotic resistance and phenotype, we remoted a reverse complement (COM) and a transconjugant of pressure FZB42 with the native pBV71. Remodeled COM and FZB42p cells have been much like the wild-type pressure GH1-13 with excessive antibiotic resistance and gradual progress charges on lactose in comparison with these of mucoid phenotypes. RT-PCR evaluation revealed that the expression of plasmid-encoded orphan aspartate phosphatase (pRapD) was coordinated with a brand new quorum-sensing (QS) cassette of RapF2-PhrF2 current within the chromosome of pressure GH1-13, however not in pressure FZB42.
) pOET-2 transfer plasmid (10ug) |
|
GWB-65CD85 |
GenWay Biotech |
0.01 mg |
Ask for price |
) pOET-4 transfer plasmid (10ug) |
|
GWB-282B50 |
GenWay Biotech |
0.01 mg |
Ask for price |
|
|
) pOET-3 transfer plasmid (10ug) |
|
GWB-23143B |
GenWay Biotech |
0.01 mg |
Ask for price |
|
|
 pOET 2 N/C_6xHis™ Transfer Vector |
|
GWB-001031 |
GenWay Biotech |
10 ug |
Ask for price |
) pOET-5 Transfer Vector (10ug) |
|
GWB-200106 |
GenWay Biotech |
10 ug |
Ask for price |
 6XHis azide |
|
12628 |
AAT Bioquest |
1mg |
EUR 301 |
|
Description: 6XHis azide is an excellent building block to make 6XHis conjugates for developing His tag detection probes and purification tools through the well-known click chemistry. |
6XHis azide |
|
12628-1mg |
AAT Bioquest |
1 mg |
EUR 295 |
|
|
|
Description: 6XHis azide is an excellent building block to make 6XHis conjugates for developing His tag detection probes and purification tools through the well-known click chemistry. |
 6XHis alkyne |
|
12629-1mg |
AAT Bioquest |
1 mg |
EUR 295 |
|
|
|
Description: 6XHis alkyne is an excellent building block to make 6XHis conjugates for developing His tag detection probes and purification tools through the well-known click chemistry. |
 6XHis maleimide |
|
12626-1mg |
AAT Bioquest |
1 mg |
EUR 295 |
|
|
|
Description: 6XHis maleimide is an excellent building block to make 6XHis conjugates for developing His tag detection probes and purification tools. |
 pOET Sequencing Primers |
|
GWB-200100 |
GenWay Biotech |
2 x 100 ul |
Ask for price |
) PLTP (Plasma Phospholipid Transfer Protein) |
|
MBS613178-02mL |
MyBiosource |
0.2mL |
EUR 650 |
PLTP (Plasma Phospholipid Transfer Protein) |
|
MBS613178-5x02mL |
MyBiosource |
5x0.2mL |
EUR 2765 |
 6XHis Succinimidyl Ester |
|
12624 |
AAT Bioquest |
1mg |
EUR 301 |
|
Description: 6XHis Succinimidyl Ester is an excellent building block to make 6XHis conjugates for developing His tag detection probes and purification tools. |
6XHis Succinimidyl Ester |
|
12624-1mg |
AAT Bioquest |
1 mg |
EUR 295 |
|
|
|
Description: 6XHis Succinimidyl Ester is an excellent building block to make 6XHis conjugates for developing His tag detection probes and purification tools. |
 6xHis Conjugated Antibody |
|
MBS9446006-01mLAF350 |
MyBiosource |
0.1mL(AF350) |
EUR 480 |
6xHis Conjugated Antibody |
|
MBS9446006-01mLAF405 |
MyBiosource |
0.1mL(AF405) |
EUR 480 |
6xHis Conjugated Antibody |
|
MBS9446006-01mLAF488 |
MyBiosource |
0.1mL(AF488) |
EUR 480 |
6xHis Conjugated Antibody |
|
MBS9446006-01mLAF555 |
MyBiosource |
0.1mL(AF555) |
EUR 480 |
6xHis Conjugated Antibody |
|
MBS9446006-01mLBiotin |
MyBiosource |
0.1mL(Biotin) |
EUR 480 |
) His-tag | 6xHis (polyclonal) |
|
AS19-4255 |
Agrisera AB |
100 µg |
EUR 312 |
 Antibody) Histidine (6xHis-tag) Antibody |
|
MBS5680102-1mg |
MyBiosource |
1mg |
EUR 515 |
Histidine (6xHis-tag) Antibody |
|
MBS5680102-5x1mg |
MyBiosource |
5x1mg |
EUR 2110 |
 (Azide Free)) PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) |
|
MBS633130-01mg |
MyBiosource |
0.1mg |
EUR 630 |
PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) |
|
MBS633130-5x01mg |
MyBiosource |
5x0.1mg |
EUR 2680 |
) His-tag | 6xHis (100 µg, Clone AD1,1,10) |
|
AS15-2937 |
Agrisera AB |
100 µg |
EUR 443 |
 N-terminal 6xHis-B2M-tag |
|
MBS7046830-1mg |
MyBiosource |
1mg |
EUR 230 |
 N-terminal 6xHis-GST-tag |
|
MBS7046838-INQUIRE |
MyBiosource |
INQUIRE |
Ask for price |
 N-terminal 6xHis-Trx-tag |
|
MBS7046849-INQUIRE |
MyBiosource |
INQUIRE |
Ask for price |
 N-terminal 6xHis-SUMO-tag |
|
MBS7046828-1mg |
MyBiosource |
1mg |
EUR 230 |
 (AP)) PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (AP) |
|
MBS6431996-01mL |
MyBiosource |
0.1mL |
EUR 950 |
PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (AP) |
|
MBS6431996-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4120 |
 (PE)) PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (PE) |
|
MBS6432006-01mL |
MyBiosource |
0.1mL |
EUR 950 |
PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (PE) |
|
MBS6432006-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4120 |
 (APC)) PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (APC) |
|
MBS6431997-01mL |
MyBiosource |
0.1mL |
EUR 950 |
PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (APC) |
|
MBS6431997-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4120 |
 (HRP)) PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (HRP) |
|
MBS6432000-01mL |
MyBiosource |
0.1mL |
EUR 950 |
PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (HRP) |
|
MBS6432000-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4120 |
 (FITC)) PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (FITC) |
|
MBS6431999-01mL |
MyBiosource |
0.1mL |
EUR 950 |
PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (FITC) |
|
MBS6431999-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4120 |
 (Biotin)) PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (Biotin) |
|
MBS6431998-01mL |
MyBiosource |
0.1mL |
EUR 950 |
PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (Biotin) |
|
MBS6431998-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4120 |
) MsrB2 Protein aa: 42-182 (6xHis-tag) |
|
MB201 |
Cytoskeleton |
2 x 50 µg |
EUR 340.08 |
MsrB2 Protein aa: 42-182 (6xHis-tag) |
|
MB201-XL |
Cytoskeleton |
1 x 1 mg |
EUR 1490.32 |
) LCK with C-tGFP tag for Plasma memberane marking (10ug transfection-grade plasmid) |
|
RC100017 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) LCK with C-tRFP tag for Plasma memberane marking (10ug transfection-grade plasmid) |
|
RC100049 |
Origene Technologies GmbH |
10 µg |
Ask for price |
 (Azide Free) (MaxLight 490)) PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 490) |
|
MBS6199128-01mL |
MyBiosource |
0.1(mL |
EUR 875 |
PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 490) |
|
MBS6199128-5x01mL |
MyBiosource |
5x0.1mL |
EUR 3800 |
 (Azide Free) (MaxLight 550)) PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 550) |
|
MBS6209803-01mL |
MyBiosource |
0.1(mL |
EUR 875 |
PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 550) |
|
MBS6209803-5x01mL |
MyBiosource |
5x0.1mL |
EUR 3800 |
 (Azide Free) (MaxLight 405)) PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 405) |
|
MBS6188453-01mL |
MyBiosource |
0.1(mL |
EUR 875 |
PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 405) |
|
MBS6188453-5x01mL |
MyBiosource |
5x0.1mL |
EUR 3800 |
 (Azide Free) (MaxLight 750)) PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 750) |
|
MBS6231153-01mL |
MyBiosource |
0.1(mL |
EUR 875 |
PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 750) |
|
MBS6231153-5x01mL |
MyBiosource |
5x0.1mL |
EUR 3800 |
 (Azide Free) (MaxLight 650)) PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 650) |
|
MBS6220478-01mL |
MyBiosource |
0.1(mL |
EUR 875 |
PLTP (Plasma Phospholipid Transfer Protein) (Azide Free) (MaxLight 650) |
|
MBS6220478-5x01mL |
MyBiosource |
5x0.1mL |
EUR 3800 |
 N-terminal 6xHis-B2M-JD-tag |
|
MBS7046834-INQUIRE |
MyBiosource |
INQUIRE |
Ask for price |
) His-tag | 6xHis (monoclonal, Clone 2C5,1) |
|
AS20-4457 |
Agrisera AB |
100 µg |
EUR 367 |
) CCND1 with C-tGFP tag for Nucleus marking (10ug transfection-grade plasmid) |
|
RC100009 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) LAMP1 with C-tGFP tag for Lysosome marking (10ug transfection-grade plasmid) |
|
RC100016 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) LMNB1 with N-tGFP tag for Nucleus marking (10ug transfection-grade plasmid) |
|
RC100018 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) Rab4 with N-tGFP tag for Endosome marking (10ug transfection-grade plasmid) |
|
RC100025 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) Rab5 with N-tGFP tag for Endosome marking (10ug transfection-grade plasmid) |
|
RC100026 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) RhoB with N-tGFP tag for Endosome marking (10ug transfection-grade plasmid) |
|
RC100027 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) CCND1 with C-tRFP tag for Nucleus marking (10ug transfection-grade plasmid) |
|
RC100041 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) LAMP1 with C-tRFP tag for Lysosome marking (10ug transfection-grade plasmid) |
|
RC100048 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) LMNB1 with N-tRFP tag for Nucleus marking (10ug transfection-grade plasmid) |
|
RC100050 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) Rab4 with N-tRFP tag for Endosome marking (10ug transfection-grade plasmid) |
|
RC100057 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) Rab5 with N-tRFP tag for Endosome marking (10ug transfection-grade plasmid) |
|
RC100058 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) RhoB with N-tRFP tag for Endosome marking (10ug transfection-grade plasmid) |
|
RC100059 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) LAMP1 with C-mGFP tag for Lysosome marking (10ug transfection-grade plasmid) |
|
RC100089 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) CCND1 with C-mGFP tag for Nucleus marking (10ug transfection-grade plasmid) |
|
RC100094 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) LMNB1 with N-mGFP tag for Nucleus marking (10ug transfection-grade plasmid) |
|
RC100095 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) GBA2 with C-mGFP tag for Microsome marking (10ug transfection-grade plasmid) |
|
RC100099 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) LAMP1 with C-mRFP tag for Lysosome marking (10ug transfection-grade plasmid) |
|
RC100123 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) CCND1 with C-mRFP tag for Nucleus marking (10ug transfection-grade plasmid) |
|
RC100128 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) LMNB1 with N-mRFP tag for Nucleus marking (10ug transfection-grade plasmid) |
|
RC100129 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) GBA2 with C-mRFP tag for Microsome marking (10ug transfection-grade plasmid) |
|
RC100133 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) LAMP1 with C-mBFP tag for Lysosome marking (10ug transfection-grade plasmid) |
|
RC100157 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) CCND1 with C-mBFP tag for Nucleus marking (10ug transfection-grade plasmid) |
|
RC100162 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) LMNB1 with N-mBFP tag for Nucleus marking (10ug transfection-grade plasmid) |
|
RC100163 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) GBA2 with C-mBFP tag for Microsome marking (10ug transfection-grade plasmid) |
|
RC100167 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) ExoFectin® Plasmid DNA-into-Exosome Kit (DNA transfection kit for exosome) |
|
P402 |
101Bio |
20 rxn |
EUR 499 |
) CLTB with N-tGFP tag for Coated pit marking (10ug transfection-grade plasmid) |
|
RC100010 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) CLTB with N-tRFP tag for Coated pit marking (10ug transfection-grade plasmid) |
|
RC100042 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) CLTB with N-mGFP tag for Coated Pit marking (10ug transfection-grade plasmid) |
|
RC100071 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) CLTB with N-mRFP tag for Coated Pit marking (10ug transfection-grade plasmid) |
|
RC100105 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) CLTB with N-mBFP tag for Coated Pit marking (10ug transfection-grade plasmid) |
|
RC100139 |
Origene Technologies GmbH |
10 µg |
Ask for price |
 (MaxLight 405)) PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 405) |
|
MBS6432001-01mL |
MyBiosource |
0.1mL |
EUR 950 |
PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 405) |
|
MBS6432001-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4120 |
 (MaxLight 490)) PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 490) |
|
MBS6432002-01mL |
MyBiosource |
0.1mL |
EUR 950 |
PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 490) |
|
MBS6432002-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4120 |
 (MaxLight 550)) PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 550) |
|
MBS6432003-01mL |
MyBiosource |
0.1mL |
EUR 950 |
PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 550) |
|
MBS6432003-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4120 |
 (MaxLight 650)) PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 650) |
|
MBS6432004-01mL |
MyBiosource |
0.1mL |
EUR 950 |
PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 650) |
|
MBS6432004-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4120 |
 (MaxLight 750)) PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 750) |
|
MBS6432005-01mL |
MyBiosource |
0.1mL |
EUR 950 |
PLTP (Plasma Phospholipid Transfer Protein, BPIFE, HDLCQ9) (MaxLight 750) |
|
MBS6432005-5x01mL |
MyBiosource |
5x0.1mL |
EUR 4120 |
-Human cholesteryl ester transfer protein, plasma (CETP)) CETP (untagged)-Human cholesteryl ester transfer protein, plasma (CETP) |
|
SC124106 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) BID with C-tGFP tag for Mitochondria marking (10ug transfection-grade plasmid) |
|
RC100007 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) PLK1 with N-tGFP tag for Centrosome marking (10ug transfection-grade plasmid) |
|
RC100023 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) PXMP2 with N-tGFP tag for Peroxisome marking (10ug transfection-grade plasmid) |
|
RC100024 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) BID with C-tRFP tag for Mitochondria marking (10ug transfection-grade plasmid) |
|
RC100039 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) PLK1 with N-tRFP tag for Centrosome marking (10ug transfection-grade plasmid) |
|
RC100055 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) PXMP2 with N-tRFP tag for Peroxisome marking (10ug transfection-grade plasmid) |
|
RC100056 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) PLK1 with N-mGFP tag for Centrosome marking (10ug transfection-grade plasmid) |
|
RC100070 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) BID with C-mGFP tag for Mitochondria marking (10ug transfection-grade plasmid) |
|
RC100090 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) PLK1 with N-mRFP tag for Centrosome marking (10ug transfection-grade plasmid) |
|
RC100104 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) BID with C-mRFP tag for Mitochondria marking (10ug transfection-grade plasmid) |
|
RC100124 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) PLK1 with N-mBFP tag for Centrosome marking (10ug transfection-grade plasmid) |
|
RC100138 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) BID with C-mBFP tag for Mitochondria marking (10ug transfection-grade plasmid) |
|
RC100158 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) His-tag | 6xHis (clone HIS,H8 / EH158) |
|
AS11-1771 |
Agrisera AB |
50 µg |
EUR 259 |
 H-Ras Protein; wild type 6xHis Tag |
|
RS01-A |
Cytoskeleton |
1 x 100 µg |
EUR 469.04 |
H-Ras Protein; wild type 6xHis Tag |
|
RS01-C |
Cytoskeleton |
3 x 100 µg |
EUR 836.16 |
) ACTB with N-tGFP tag for Cytoskeleton marking (10ug transfection-grade plasmid) |
|
RC100002 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) PDHA1 with C-tGFP tag for Mitochondria marking (10ug transfection-grade plasmid) |
|
RC100006 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) PFN1 with N-tGFP tag for Cytoskeleton marking (10ug transfection-grade plasmid) |
|
RC100022 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) PDHA1 with C-tRFP tag for Mitochondria marking (10ug transfection-grade plasmid) |
|
RC100038 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) PFN1 with N-tRFP tag for Cytoskeleton marking (10ug transfection-grade plasmid) |
|
RC100054 |
Origene Technologies GmbH |
10 µg |
Ask for price |
) ATG12 with C-mGFP tag for Auophagasome marking (10ug transfection-grade plasmid) |
|
RC100066 |
Origene Technologies GmbH |
10 µg |
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) PDHA1 with C-mGFP tag for Mitochondria marking (10ug transfection-grade plasmid) |
|
RC100091 |
Origene Technologies GmbH |
10 µg |
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) ATG12 with C-mRFP tag for Auophagasome marking (10ug transfection-grade plasmid) |
|
RC100100 |
Origene Technologies GmbH |
10 µg |
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) PDHA1 with C-mRFP tag for Mitochondria marking (10ug transfection-grade plasmid) |
|
RC100125 |
Origene Technologies GmbH |
10 µg |
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) ATG12 with C-mBFP tag for Auophagasome marking (10ug transfection-grade plasmid) |
|
RC100134 |
Origene Technologies GmbH |
10 µg |
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) PDHA1 with C-mBFP tag for Mitochondria marking (10ug transfection-grade plasmid) |
|
RC100159 |
Origene Technologies GmbH |
10 µg |
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) GAP43 with C-tGFP tag for Neuroal axis marking (10ug transfection-grade plasmid) |
|
RC100013 |
Origene Technologies GmbH |
10 µg |
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) GAP43 with C-tRFP tag for Neuroal axis marking (10ug transfection-grade plasmid) |
|
RC100045 |
Origene Technologies GmbH |
10 µg |
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) RhoB with N-mGFP tag for Early Endosome marking (10ug transfection-grade plasmid) |
|
RC100075 |
Origene Technologies GmbH |
10 µg |
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) Rab4 with N-mGFP tag for Early Endosome marking (10ug transfection-grade plasmid) |
|
RC100077 |
Origene Technologies GmbH |
10 µg |
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) Rab5 with N-mGFP tag for Early Endosome marking (10ug transfection-grade plasmid) |
|
RC100078 |
Origene Technologies GmbH |
10 µg |
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) RhoB with N-mRFP tag for Early Endosome marking (10ug transfection-grade plasmid) |
|
RC100109 |
Origene Technologies GmbH |
10 µg |
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) Rab4 with N-mRFP tag for Early Endosome marking (10ug transfection-grade plasmid) |
|
RC100111 |
Origene Technologies GmbH |
10 µg |
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) Rab5 with N-mRFP tag for Early Endosome marking (10ug transfection-grade plasmid) |
|
RC100112 |
Origene Technologies GmbH |
10 µg |
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) RhoB with N-mBFP tag for Early Endosome marking (10ug transfection-grade plasmid) |
|
RC100143 |
Origene Technologies GmbH |
10 µg |
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) Rab4 with N-mBFP tag for Early Endosome marking (10ug transfection-grade plasmid) |
|
RC100145 |
Origene Technologies GmbH |
10 µg |
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) Rab5 with N-mBFP tag for Early Endosome marking (10ug transfection-grade plasmid) |
|
RC100146 |
Origene Technologies GmbH |
10 µg |
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 - Human cholesteryl ester transfer protein, plasma (CETP)) CETP (GFP-tagged) - Human cholesteryl ester transfer protein, plasma (CETP) |
|
RG206561 |
Origene Technologies GmbH |
10 µg |
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) ATG12 with C-tGFP tag for Autophagosome marking (10ug transfection-grade plasmid) |
|
RC100004 |
Origene Technologies GmbH |
10 µg |
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) MAPRE3 with C-tGFP tag for Cytoskeleton marking (10ug transfection-grade plasmid) |
|
RC100019 |
Origene Technologies GmbH |
10 µg |
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) TUBA1B with N-tGFP tag for Cytoskeleton marking (10ug transfection-grade plasmid) |
|
RC100030 |
Origene Technologies GmbH |
10 µg |
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) ATG12 with C-tRFP tag for Autophagosome marking (10ug transfection-grade plasmid) |
|
RC100036 |
Origene Technologies GmbH |
10 µg |
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) MAPRE3 with C-tRFP tag for Cytoskeleton marking (10ug transfection-grade plasmid) |
|
RC100051 |
Origene Technologies GmbH |
10 µg |
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) MAPRE3 with C-mGFP tag for Cytoskeleton marking (10ug transfection-grade plasmid) |
|
RC100072 |
Origene Technologies GmbH |
10 µg |
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) MAPRE3 with C-mRFP tag for Cytoskeleton marking (10ug transfection-grade plasmid) |
|
RC100106 |
Origene Technologies GmbH |
10 µg |
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) MAPRE3 with C-mBFP tag for Cytoskeleton marking (10ug transfection-grade plasmid) |
|
RC100140 |
Origene Technologies GmbH |
10 µg |
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Multi-omics evaluation on wild-type and plasmid-cured cells of pressure GH1-13 steered that the conjugative plasmid expression has an important position in induction of early envelope stress response that promotes cell morphogenesis, biofilm formation, catabolite repression, and biosynthesis of extracellular-matrix elements and antibiotics for defense of host cell throughout exponential part.
