nNOS:C-Terminal (neuronal Nitric Oxide Synthase) Antibody

Biotin Blog

The ImmunoStar neuronal nitric oxide synthase C-terminal antiserum was quality control tried utilizing standard immunohistochemical strategies. The antiserum shows unequivocally sure marking of rodent nerve center, striatum, cortex and spinal string utilizing roundabout immunofluorescent and biotin/avidin-HRP strategies. Suggested essential weakening is 1/8,000 – 1/12,000 in PBS/0.3% Triton X-100 – Bn/Av-HRP Technique.

By Western blotch examination of cerebrum homogenates the immunizer explicitly names a band of roughly 155 kD. Immuno-marking is totally annulled by pre-adsorption with manufactured human nNOS (1419-1433) at 5 µg per mL of weakened counter acting agent. No cross reactivity with different types of NOS were noticed.

Photograph Description

IHC picture of a solitary neuron staining for the C-terminal of nNOS in the rodent cortex (above), alongside low amplification of cortex (underneath left), neurons in the rodent dentate gyrus (beneath center) and in the striatum (underneath right).

The tissue was fixed with 4% formaldehyde in phosphate cushion, prior to being eliminated and ready for vibratome segment. Drifting segments were hatched at RT in 10% goat serum in PBS, before standard IHC system. Essential neutralizer was brooded at 1:10000 for 48 hours, goat hostile to bunny optional was therefore added for 1 hour subsequent to washing with PBS. Light microscopy staining was accomplished with standard biotin-streptavidin/HRP method and DAB chromogen, underneath center picture with nickel stain.

nNOS Antibodies

Antibodies that distinguish nNOS can be utilized in a few logical applications, including Western Blot, Immunohistochemistry (Paraffin), Immunocytochemistry, ELISA and Immunohistochemistry (Frozen). These antibodies target nNOS in Human, Rat, Mouse, Rabbit and Guinea Pig tests. Our nNOS polyclonal, recombinant monoclonal and monoclonal antibodies are created in Rabbit, Goat and Mouse.

These antibodies have been checked by Cell treatment and Relative articulation to affirm explicitness to nNOS. Track down the nNOS counter acting agent that meets your requirements. Browse 1 of 17 nNOS antibodies, which have been approved in tries different things with 176 distributions and 119 pictures highlighted in our information display.

Peruse essential antibodies for WB, Flow, IHC, ICC/IF, ELISA, IP, and different applications. Antibodies with Advanced Verification information have been approved for explicitness to guarantee that the immune response ties to the antigen expressed. On the off chance that you can’t observe the counter acting agent you’re searching for, reach us today to foster custom antibodies for explicit targets, species and applications.

Target Information

NOS action requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic gatherings (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). A few unmistakable NOS isoforms have been portrayed and been displayed to address the results of three particular qualities. These incorporate two constitutive Ca2+/CaM-subordinate types of NOS, including NOS1 (likewise assigned ncNOS) whose movement was first distinguished in quite a while, and NOS3 (additionally assigned ecNOS), first recognized in quite a while.

Equivalents

BNOS; bNOS1; Brain NOS; Constitutive NOS; N-NOS; NC-NOS; Neuronal NOS; nitric oxidase synthase; nitric oxide synthase 1 (neuronal); Nitric oxide synthase, cerebrum; nNOS; nNOS:C-Terminal (neuronal Nitric Oxide Synthase)

Antibody

The neuronal nitric oxide synthase C-terminal antiserum was quality control tried utilizing standard immunohistochemical techniques. The antiserum shows emphatically certain marking of rodent nerve center, striatum, cortex and spinal line utilizing backhanded immunofluorescent and biotin/avidin-HRP strategies. Suggested essential weakenings are 1/1,000 – 1/1,500 in PBS/0.3% Triton X-100 – Cy3 Technique and 1/8,000 – 1/12,000 in PBS/0.3% Triton X-100 – Bn/Av-HRP Technique.

nNOS (Neuronal Nitric Oxide Synthase)

RA19011 Neuromics 100 uL 451.2 EUR

Human Carboxyl- terminal PDZ ligand of neuronal nitric oxide syn

ELI-33099h Lifescience Market 96 Tests 988.8 EUR

Rat Carboxyl- terminal PDZ ligand of neuronal nitric oxide synth

ELI-10703r Lifescience Market 96 Tests 1063.2 EUR

Mouse Carboxyl- terminal PDZ ligand of neuronal nitric oxide syn

ELI-10812m Lifescience Market 96 Tests 1038 EUR

Neuronal Nitric Oxide Synthase (Nnos) Antibody

20-abx114082 Abbexa
  • 878.40 EUR
  • 477.60 EUR
  • 150 ul
  • 50 ul

Human Neuronal Nitric Oxide Synthase ELISA kit

E01N0069-192T BlueGene 192 tests 1524 EUR

Human Neuronal Nitric Oxide Synthase ELISA kit

E01N0069-48 BlueGene 1 plate of 48 wells 624 EUR

Human Neuronal Nitric Oxide Synthase ELISA kit

E01N0069-96 BlueGene 1 plate of 96 wells 822 EUR

Goat Neuronal Nitric Oxide Synthase ELISA kit

E06N0069-192T BlueGene 192 tests 1524 EUR

Goat Neuronal Nitric Oxide Synthase ELISA kit

E06N0069-48 BlueGene 1 plate of 48 wells 624 EUR

Goat Neuronal Nitric Oxide Synthase ELISA kit

E06N0069-96 BlueGene 1 plate of 96 wells 822 EUR

Dog Neuronal Nitric Oxide Synthase ELISA kit

E08N0069-192T BlueGene 192 tests 1524 EUR

Dog Neuronal Nitric Oxide Synthase ELISA kit

E08N0069-48 BlueGene 1 plate of 48 wells 624 EUR

Dog Neuronal Nitric Oxide Synthase ELISA kit

E08N0069-96 BlueGene 1 plate of 96 wells 822 EUR

Rat Neuronal Nitric Oxide Synthase ELISA kit

E02N0069-192T BlueGene 192 tests 1524 EUR

Rat Neuronal Nitric Oxide Synthase ELISA kit

E02N0069-48 BlueGene 1 plate of 48 wells 624 EUR

Rat Neuronal Nitric Oxide Synthase ELISA kit

E02N0069-96 BlueGene 1 plate of 96 wells 822 EUR

Mouse Neuronal Nitric Oxide Synthase ELISA kit

E03N0069-192T BlueGene 192 tests 1524 EUR

Mouse Neuronal Nitric Oxide Synthase ELISA kit

E03N0069-48 BlueGene 1 plate of 48 wells 624 EUR

By Western smudge examination of cerebrum homogenates the immune response explicitly marks a band of around 155 kD. Immuno-naming is totally nullified by pre-adsorption with manufactured human nNOS (1419-1433) at 5 µg per mL of weakened immunizer. No cross reactivity with different types of NOS were noticed. The nNOS antiserum has been utilized effectively in human, rodent, mouse, guinea pig, feline, and monkey tissue. Discovery of nNOS from different species will rely on arrangement homology.

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