InstantBlue was utilized in Coomassie staining to approve the presence of proteins from SDS-PAGE gels. It was additionally used to color SDS-PAGE gels.
Depiction
is a prepared to-utilize arrangement uncommonly figured out for super quick (under 15 minutes), delicate (5 ng for each band (BSA)) and safe identification of your proteins. Protein gels can be colored with InstantBlue in minutes without washing, fixing or destacking. Gels can stay colored for a really long time without issues. Just proteins are stained, bringing about plainly characterized blue groups on an exceptionally straightforward foundation.
Why pick Instant blue?
Lessening foundation clamor prompts a superior sign to-commotion proportion
General goal and responsiveness.
The plan of InstantBlue is non-poisonous and contains no methanol.
Proteins stained with InstantBlue staining are additionally viable with mass spectrometry (MS) examination.
Super quick staining: brings about 15 minutes or less
One stage strategy
- High awareness: 5 ng groups perceptible
- Adaptable
- Quantitative: consistency from one group to another
- Productive: just 25 ml for every gel
- Safe structure
- Methanol free.
InstantBlue is a prepared to-utilize Coomassie protein stain for polyacrylamide gels. Its interesting instrument of activity stains proteins quickly and leaves an unmistakable foundation, taking out the requirement for fixing, washing or destacking.
Data
Super quick coloring and one-venture process
High responsiveness with noticeable 5 ng groups
Clear foundation with high sign to-clamor proportion
Formed for safe use and simple removal
Accessible in a reasonable 1 liter premixed volume
The novel licensed system starts to variety in a brief time frame, while some customary pre-assembled and natively constructed Coomassie R-250 stains require three hours or more to variety gels totally. An acidic corrosive free detailing contains no methyl or acetylate proteins during staining, making it completely viable with mass spectrometry.
Likewise, because of its without methanol creation, the stain is non-poisonous and dispensable and doesn’t need microwaves, dissolvable removal or smoke hoods.
Content:
1L reagent containing Coomassie color, ethanol, phosphoric corrosive and solubilizer
Substances in water (Attention: Phosphoric corrosive is a destructive fluid.)
Capacity:
After receipt, store at + 4°C. Dispose of all reagents showing staining or
Note on microbial tainting, make certain to keep the jug shut if not
in real life.
Super FAST, 1-STEP PROTEIN GEL STAINING.
InstantBlue is a prepared to-utilize protein stain for polyacrylamide gels. Its one of a kind component of activity stains proteins in a short time and leaves an unmistakable foundation, wiping out the need to fix, wash or weaken them. InstantBlue is planned for safe use and simple removal.
InstantBlue 1L (Instant Blue) |
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