A Novel 2A-peptide-containing Plasmid to Generate Stable Perkinsus marinus Cells Expressing Organelle-targeted Genes

A Novel 2A-peptide-containing Plasmid to Generate Stable Perkinsus marinus Cells Expressing Organelle-targeted Genes

Genetic manipulation methods for marine protists will not be well-established regardless of immense efforts. Nonetheless, Perkinsus marinus is an exception, and will be developed as a genetically tractable mannequin organism for associated protists. Right here, we designed a brand new plasmid for P. marinus that enables two proteins from a single mRNA to be in […]

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Two Plasmid-Based Systems for CRISPR/Cas9 Mediated Knockout of Target Genes

Two Plasmid-Based Systems for CRISPR/Cas9 Mediated Knockout of Target Genes

CRISPR/Cas9-based gene modifying is a current advance that enables for the knockout or alteration of goal genes inside mammalian cells. Many variations of the method exist, however right here we describe two programs of plasmid-based CRISPR gene knockout which collectively permit for the selective knockout of nearly any gene goal. In contrast with different CRISPR-based […]

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Docetaxel >99%

CAS: 114977-28-5 Molecular formula: C43H53NO14 Molecular weight (g / mol): 807.89 MDL Number: MFCD00800737 InChI key: ZDZOTLJHXYCWBA-VCVYQWHSSA-N Synonymous: docetaxel, Taxotere, anhydrous docetaxel, docetaxel, emdoc, docetaxel, trihydrate, docetaxel inn, Taxotere tn, unit-699121phca, xl PubChem CID: 148124 CHEBI CHIBI: 4672 Smiles: CC1 = C2C (C (= O) C3 (C (CC4C (C3C (C (C2 (C) C) C) (CC1OC […]

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Rapamycine 100mg

Description Rapamycin (AY-22989, Rapamune, Sirolimus, NSC-2260804) is a specific mTOR inhibitor with an IC50 of ~ 0.1 nM for HEK293 cells. Goals mTOR (HEK293 cells) ~ 0.1 nM In vitro Rapamycin inhibits endogenous mTOR activity in HEK293 cells with IC50 of ~ 0.1 nM, more potent than iRap and AP21967 with IC50 of ~ 5 […]

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S. aureus alpha hemolysin (a-Toxin

Properties Quality level: 300 Contains: sodium phosphate buffer as balance Composition: Protein, ~ 25% Lowry Availability: not available in the US. Impurities Staphylococcal enterotoxin A (SEA), essentially free Storage temperature: 2-8 ° C Description Request Staphylococcal enterotoxin B (SEB) binds to MHC class II molecules and specific Vβ regions of T cell receptors (TCRs), resulting […]

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QuantiChrom™ Calcium Assay Kit 500

Quality level: 200 Qualification: for molecular biology Sterility: sterile; 0.2 μm filtered Form: solution Use kit enough for 160 transfections (6 cm dishes) kit enough for 400 transfections (3.5 cm dishes) kit enough for 80 transfections (10 cm dishes) Application(s): transfection: adequate Sent in: dry ice Storage temperature: −20 ° C General description Calcium phosphate […]

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FD Rapid GolgiStain Kit

Product name: Golgi Staining Kit – Green Fluorescence – Cytopainter Example type: Adherent cells, Cells in suspension Test type: Cell-based Species reactivity: Reacts with: Mammals, Other species Product description Golgi Staining Kit – Green Fluorescence | Cytopainter (ab139483) contains a selective Golgi stain suitable for live cells or aldehyde-fixed cell staining. Micromolar concentrations of the […]

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